Reference solution, Chemistry

Reference solution:


A proper reference solution contains color reagent plus sample buffer. The difference between the reference and a sample is that the concentration of the assayable substance in the reference solution is zero. The reference tube transmits as much light as is possible with the assay solution you are using. A sample tube with any concentration of the assayable substance absorbs more light than the reference, transmitting less light to the photometer. In order to obtain the best readability and accuracy, the scale is set to read zero absorbance (100% transmission) with the reference in place. Now you can use the full scale of the spectrophotometer. If you use a water blank as a reference, you might find that the assay solution alone absorbs so much light relative to distilled water that the usable scale is compressed, and the accuracy is very poor.


Posted Date: 10/15/2012 5:47:33 AM | Location : United States

Related Discussions:- Reference solution, Assignment Help, Ask Question on Reference solution, Get Answer, Expert's Help, Reference solution Discussions

Write discussion on Reference solution
Your posts are moderated
Related Questions
what were the drawbacks of bohr''s atomic theory

what will you observe if you freeze water at 4 degree celcious

Which of the following has the least energy: (1) 2p                    (2)  3p            (3) 2s                             (4) 4d Ans: 2s

Lowest sum rule - iupac system of nomenclature previously it may be noted that, the numbering of the parent chain consisting of two or more substituents was done in such a way

Q.  Ca +2 = 20 ppm, Mg +2 = 25 ppm, CO 2 = 30 ppm, HCO - 3 = 150, K + = 10 ppm. Calculate the lime (87% pure) and soda (91% pure) required softening 1 million litt

Benzyne - organic chemistry (1) 1, 2-Didehydrobenzene, C 6 H 6 and its derivatives are known as benzyne or arynes and the simplest member is benzyne.      (2) It is neutral re

how to determine the concentration of citric acid given that it reacts with sodium hydroxide

how we synthesis picric acid why it required 1 hour for the reaction with HNO3,H2SO4 and show the mechanism of picric acid and uses of picric acid. and what is the role of HNO3,H2S