Radioactive labelling, Biology

Radioactive Labelling

Radioactive labelling method has been effectively applied on the chick blastoderm. The method includes labelling one embryo (donor) and grafting a part of it in similar position on another unlabelled embryo of similar stage (Host). An explanted blastodermis immersed in a medium consisting of the radioactive mtiated (H3) thymidine. In 3 to eight hours the tritiated thymidine is incorporated into the chromosomal DNA of dividing blastodermal cells. The embryo labelled in such type of a way by tritiated thymidine serves as a donor. Another embryo at similar stage of development as attained through the labelled donor in the meantime is then chosen to serve as the host. A small area of the host embryo is excised and replaced by a corresponding piece from the donor of which the fate is to be determined. Healing generally takes place quickly and the development is not impaired if the operation has been done carefully.

The thymidine does not pass out of the nuclei of the labelled cells but stays in the chromosomes of their descendents. Even though the radioactive thymidine present in the DNA is gradually diluted with each subsequent chromosomal replication radioactivity remains for a considerable time. Such type of composite embryo (partly from donor and partly from the host) is tested at a later stage of development for radioactivity through special techniques like autoradiography etc. Only the part(s) or structure(s) developed from the grafted piece display the presence of radioactivity thus establishing the fate of specific area taken from the donor.

Posted Date: 1/25/2013 4:41:35 AM | Location : United States







Related Discussions:- Radioactive labelling, Assignment Help, Ask Question on Radioactive labelling, Get Answer, Expert's Help, Radioactive labelling Discussions

Write discussion on Radioactive labelling
Your posts are moderated
Related Questions
Q. Can you define Regulation of Coronary Blood flow? During diastole when the aortic valve (AV) is closed, aortic diastolic pressure is transmitted through the dilated sinuses

hows does insulin from the pancreas reach the liver?

Aerobic Exercises, Examples and their Do's and Don'ts In the aerobic exercises it requires activities performed for a longer period here we do like to mention certain activitie

Definition of osmosis A) The best explanation of osmosis is 'The movement of water from a dilute solution to a    concentrated solution across a partially permeable membrane'.

What are three main problems with having only two kingdoms There are three main problems with having only two kingdoms. The first problem concerns the unicellular flagellates

Gibberellins - Plant Growth Substances In late 1920's Kurosawa observed that the medium in which the fungus Gibberella had grown could cause the abnormal tallness in rice seed

State the basic properties of Cell The differences of cell types notwithstanding, the similarities among them are more profound. Actually, all cells possess the same basic prop

Nutrition and Health The effects of the socio-economic and environmental factors in which one is constrained to live will manifest in terms of the nutritional and health statu

do you have college level developmental biologists available

Distinguish features in the mechanisms of RNA synthesis, + (RNA), (-) RNA, and ds RNA viruses.