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Explain the Process of dna technology
Procedures involving recombinant DNA technology has been used to increase the yield of enzymes already produced by the microorganisms, e.g., β-galactosidase by E. coli or to produce completely different enzymes including ones normally synthesized by eukaryotic cells. These techniques may even be used to produce enzymes of modified structure from suitably modified or synthesized genes. This is termed as protein engineering. Once a suitable strain of a potential microorganism has been identified, the same may be grown to produce larger amounts of enzyme. Most enzymes obtained commercially from microbial fermentation procedures are hydrolases. These are usually extracellular enzymes.
Define Reagents for Determination of the Saponification Number of Fats? 0.5 N alcoholic KOH 0.5 N HCI Solid sodium carbonate 1% alcoholic solution of methyl orange
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