Procedures for diagnosis - nucleic acid hybridization, Biology

Nucleic acid hybridization:

This is a process by which a suitably labeled DNA or RNA from a known infectious agent is used to probe its complementary sequence unique to the pathogen (target) by hybridization process. Recent advances in DNA cloning and sequencing have greatly increased the usefulness of this technique in the diagnosis and epidemiology of diseases. It is now possible to make DNA and RNA probes of high specificity using isotopic and non-isotopic labels. The specific cDNA probes have been used with advantage to diagnose a number of viral, bacterial and parasitic diseases obviating the need for the isolation of the causative agent. Post- mortem samples can also be used to identify the pathogen involved in a particular disease. The use of non-radioactive labeled photobiotinylated cold probes is more adaptive for field situations. Cold probes have been used in diseases like blue-tongue and FMD. DNA probes have also been used to detect FMD virus nucleic acid sequences persisting in the oesopharyngeal fluids of bovines when infectious virus can not be isolated. Thus, nucleic acid probes could even be used to screen the animals for persistent virus infections.

Posted Date: 9/19/2012 1:30:16 AM | Location : United States







Related Discussions:- Procedures for diagnosis - nucleic acid hybridization, Assignment Help, Ask Question on Procedures for diagnosis - nucleic acid hybridization, Get Answer, Expert's Help, Procedures for diagnosis - nucleic acid hybridization Discussions

Write discussion on Procedures for diagnosis - nucleic acid hybridization
Your posts are moderated
Related Questions
Similarities  in cloning and selective breeding cloning (not transgenesis) and selective breeding both transfer whole genome both selective breeding and cloning have t

Organic Substances - Abiotic Components These include carbohydrates, proteins, lipids and their derivatives which are derived from the waste products of plants and animals or

What is mucoperiosteal flap Silk is easy to handle, ties with a slip knot, and is relatively inexpensive compared with other nonabsorbable suture materials currently available.

What does skin color in man shows?

Excretory organ for protozoa

Define Management of Canal Blockage Canal Calcification Diamond coated instrument rt 1 designed for better visibility and cutting efficiency a) Very powerful for efficient

Suspensor - Pollen Biology The structure and function of suspensor have not been given adequate attention. During the last decade, however, investigations have produced notewo

Foetal maceration and mummification Foetal mummification occurs when the foetus dies in the uterus in the absence of air and bacterial contamination and the cervix remains tig

how are the listed from largest group to smallest group

What are the benefits of increasing the growth of bifidobacteria? Bifidobacteria displaces potential pathogens selectively, showing an antibiotic like effect, which is unrelate