Principle of colorimetric method-2, 4 dinitrophenylhydrazine, Biology

Define Principle of colorimetric method-2, 4 dinitrophenylhydrazine?

Ascorbic acid is oxidized to dehydro ascorbic acid by copper (Cu++) ions. 2, 4 dinitrophenyl hydrazine reacts with the ketone group of dehydro ascorbic acid under acidic conditions to give osazone. The depth of the yellowish orange coloured osazone produced upon acidification with sulphuric acid is directly proportional to the amount of ascorbic acid present and can be measured photometrically at 540 nm wavelength.

As you carry out the procedure in the laboratory you will understand the method even better. So then let's get started and practically learn the two methods we have studied above. There are four activities in this practical.

Posted Date: 6/18/2013 1:27:36 AM | Location : United States







Related Discussions:- Principle of colorimetric method-2, 4 dinitrophenylhydrazine, Assignment Help, Ask Question on Principle of colorimetric method-2, 4 dinitrophenylhydrazine, Get Answer, Expert's Help, Principle of colorimetric method-2, 4 dinitrophenylhydrazine Discussions

Write discussion on Principle of colorimetric method-2, 4 dinitrophenylhydrazine
Your posts are moderated
Related Questions
Q. How different are hemoglobin and oxyhemoglobin? Where is it expected to find a higher concentration of oxyhemoglobin, in peripheral tissues or in the lungs? Oxygen-bou

Biological Significance of Incompatibility Both interspecific and intraspecific incompatibility determines the degree of inbreeding and out breeding of plants. Self-incompati

Explain Negative Staining Technique? Here, a stain has a negatively charged chromophore that is repelled by the negative bacterial surface. It results in indirect staining of m

Acute Myocardial Infarction :  Patients with acute non-Q myocardial infarction may need urgent intervention as indicated for cases of unstable angina.

Q. What is the climax stage of an ecological succession? The climax stage is the stage of an ecological succession in which the community of an ecosystem turn into stable and d

Q. What is the action mechanism of the antiretroviral drugs known protease inhibitors which are used against HIV infection? Protease inhibitors are some of the antiretroviral d

Why in the preparation of a karyotype analysis is the use of a substance like colchicine interesting? The Colchicine is a substance that disallows the formation of microtubules

Define Voriconazole It is metabolized in the liver by CYP2C19, CYP2C9 and CYP3A4. CYP2C19 is genetically variant (about 3% to 5% of Caucasians and African-Americans and about 1

Structure of human testis: The male reproductive system consists of a pair of testis. Testis are male reproductive organs and produce (male gametes) sperms and also secrete

In the axon of a nerve cell, A. the voltage-dependent conductance of the voltage-gated potassium channel has a faster time course than the voltage-dependent conductance of the