Preparation - culture medium, Biology

Preparation - Culture Medium

Now that you are familiar with the constituents the preparation of medium is quite simple. Several plant tissue culture media are now available commercially in the form of dry powders, containing all ingredients except growth regulators, sucrose and agar. They are very convenient to prepare media for routine maintenance of cultures. Generally concentrated stock solutions of major inorganic nutrients (200 times concentrated expressed as 20 x) micro inorganic nutrients (200 x concentrated) iron (200 x concentrated) and organic nutrients except sucrose are prepared and stored in a refrigerator at 4? c. Separate stock solutions are prepared and stored for each growth regulator by dissolving it in a minimal quantity of appropriate solvent and adjusting the final volume with distilled water.

Posted Date: 1/24/2013 4:50:08 AM | Location : United States







Related Discussions:- Preparation - culture medium, Assignment Help, Ask Question on Preparation - culture medium, Get Answer, Expert's Help, Preparation - culture medium Discussions

Write discussion on Preparation - culture medium
Your posts are moderated
Related Questions
Explain the Bioavailability of Thiamin? Thiamin is readily available from the gut from food sources (as thiamin phosphate esters). Drugs and alcohol abuse may interfere with th

Release of lysosomal enzymes from white blood cells during a local immune attack can contribute to the symptoms of inflammation. Suppose, to alleviate inflammation, you develop a d

Explain the classes of biomaterials When an artificial material is placed in the human body, tissue reacts in a variety of ways depending on the material type thereby, affectin

An mRNA molecule codifies only one type of protein? Eukaryotic cells have monocistronic mRNA, i.e., every mRNA codifies only one polypeptide chain. Prokaryotes can present poly

How is the blood typing concerning the ABO system and the Rh usually done? In the blood typing for the ABO system and the Rh system a blood sample is collected from the person

How dissimilar are the swimming strategies in osteichthyes and in chondrichthyes? Why do sharks need to agitate their body to swim while bony fishes do not? Bony fishes have a

Discuss the economic/health impact of any one species of harmful water molds.

Describe ASD closure and VSD closure in heart dieases? ASD Closures : Small ASD's can be closed directly. Others will need a pericardial patch. Piimum ASD and Sinus Venosus A

????? # 100 ??????????? #Minimum ?????? ?????

What is Enzyme assay Enzyme assay may also be used to determine whether stored plant products are suitable for use as food commodities, for e.g.  α-amylase should be present