Poly-functional enzymes, Biology

Bi-and Poly-Functional Enzymes

These enzymes are generated by genes obtained by fusion of the coding regions of two or more genes encoding various enzymes. In simple words, the strategy for generation of such enzymes is as follows. Consider gene a produces enzyme A and gene b create enzyme B, and for some reason it is desirable to bring the two enzyme activities together. The stop codon, e.g. TAG, of gene is removed, and that changed gene a is then connected with the initiation codon or TAG of gene b. It required, a linker sequence encoding 2-10 amino acids can be started between the genes b and a. It is required that the fusion of the two genes does not modify the reading frame of gene b, i.e. the two genes are refused in the general reading frame. The fused gene will give a single polypeptide which can possess/exhibit the activities of both the enzymes B and A.

 

 

 

Posted Date: 10/2/2012 1:23:40 AM | Location : United States







Related Discussions:- Poly-functional enzymes, Assignment Help, Ask Question on Poly-functional enzymes, Get Answer, Expert's Help, Poly-functional enzymes Discussions

Write discussion on Poly-functional enzymes
Your posts are moderated
Related Questions
Cleft grafting  The stem of the stock is cut across. A 'V' shaped insertion is made at the end of the stock. The scion is cut in shape. The scion is inserted into the 'V'

Q. Fish identity card. How are fishes characterized according to examples of representing beings, skin, basic morphology, circulation, nitrogen waste, respiration, thermal control

Cat scratch disease It is caused by Bortenella henselae, a gram-negative, non-motile bacterium. The organism is found in all parts of the world. The cat is an important reserv

Determine recommended nutrient intakes by selenium? The FAO/WHO 2004 recommendation for nutrient intake for selenium by groups is given in Table. How do these recommendations c

Downer cow syndrome This syndrome is a common sequel to milk fever. The term is commonly applied to those hypocalcaemic cows that remain recumbent for several hours before bein

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Determine the concept of  imitation in the learning process The concept of  imitation in the learning process is also a key developmental concept for infants and young children

what is the chemical reaction of respiration in cockroach?

what type of skull does amphibians possess in general?

What is Vacuole ? Another structure found only in plant cells is the large central vacuole. The vacuole stores enzymes and waste products, in addition to providing the turgor p