Pollen biology, Biology

Pollen Biology

Fluorescence microscopy coupled with biochemical analysis has helped resolve the differential activity of the enzyme, β-l, 3- glucanase that catalyses the dissolution of callose. The enzyme activity is relatively low during the first meiotic division but reaches its peak at the time of spore release. This sequence of events controlling the enzyme activity is important, as an early dissolution of callose may lead to sterility. Use of autoradiographic and fluorochromatic techniques demonstrated the reduced permeability of the callose wall and the resultant chemical isolation of microspores.

A combination of phase-contrast and fluorescence microscopy also revealed that the callose wall during exins formation serves as a mold or template. Factors that trigger the onset and regulation of meidsis in anther and ovule can be worked out by employing labeled reagents. Biochemical studies through isoenzyme markers have already demonstrated specific stages of meiotic process. There is an increase in the activity of acid phosphatase at zygotene, followed by subsequent decline. At pachytene, marked activation of endonucleases is recorded. Isoperoxidases serve as markers in normal pollen development in Mercurialis annua.

Posted Date: 1/24/2013 5:45:50 AM | Location : United States







Related Discussions:- Pollen biology, Assignment Help, Ask Question on Pollen biology, Get Answer, Expert's Help, Pollen biology Discussions

Write discussion on Pollen biology
Your posts are moderated
Related Questions
Q. What are the flat bones and the long bones? The main bones of the body may be classified as long or flat bones there are bones not classified into these categories. Instance

What are the kinds of plant geotropisms? Why do the root and the stem present opposite geotropisms? The kinds of geotropisms are the positive geotropism, that in which the plan

how biochemical evidence proof DNA as a genetic material?

MEIOSIS Every  organism is mortal, It  becomes  old and wornout  with advancing age  (= ageing )  and ultimately dies ,Before  death   however  ,it reproduces its own  kind, so

Define functions of magnesium - Macro minerals? Like Ca, Mg too has a role in bone formation. Soft tissue magnesium functions as a cofactor of many enzymes involved in energy m

Define the Meaning of Goitrogens? Goitrogens are substances that interfere with iodide metabolism in any way that inhibits thyroid hormone synthesis. As a result, there is augm

Megasporogenesis One of the nucellar cells situated subjacent to the epidermis differentiates into the primary archesporial cell. This cell is larger than the adjacent ce

A lk a l in e indigestion The disease occurs due to the consumption of excessive amount of protein rich concentrate or non protein nitrogenous substance like urea, and is

How successful are you at synthesizing genes with really high G/C content? A: We have an extremely low failure rate, which is about one out of five thousand or ten thousand gen

why does the monohybrid always result in a 1:2:1 ratio?