Phase contrast microscopy, Biology

Phase Contrast Microscopy

Phase contrast microscope has the resolving power similar to ordinary light microscope. However, it enables us to observe the cellular organelles in living cell. The working of this microscope is based on the principle of phase contrast.

To understand phase contrast microscopy you should know that whenever two light waves interfere with each other, the resultant light wave may have either greater or lesser amplitude depending on the phases of the light waves (Figure shown below).

988_phase microscope1.jpg

It is a known fact that a light wave passing through a transparent object suffers a change in phase.

The living cells are heterogeneous in their cellular components having different thickness. Due to this difference, each component has different refractive index. Hence, when a light wave passes through the cell, a change in phase is produced due to differences of thickness and refractive indices of cellular components. The phase contrast microscope exaggerates the small difference between the phases and enables one to distinguish the adjacent structures.

Posted Date: 12/10/2012 12:44:54 AM | Location : United States

Related Discussions:- Phase contrast microscopy, Assignment Help, Ask Question on Phase contrast microscopy, Get Answer, Expert's Help, Phase contrast microscopy Discussions

Write discussion on Phase contrast microscopy
Your posts are moderated
Related Questions
Q. Rhythm and conduction disturbances? Alterations in cardiac rhythm occur frequently with exercise and are important in understanding a patients function and in providing pred

Q. Which is the kind of muscle tissue that moves the bones? The bones are moved by the skeletal striated muscles these muscles are voluntary (controlled by volition).

What is the physical experiment that show that the protoplasm has contractility?

commercially important bivalve species

Define the term - lateralisation and localisation Discriminative validity studies including lateralisation and localisation achieved satisfactory results, but the localisation

What are the main biological functions in which chlorine ions participate? Like sodium cations, chlorine anions actively participate in the regulation of the osmolarity of tiss

EPIDERMIS - Ectodermal in origin. Outer part of skin. Made up of stratified epithelium. Branches of sensory nerves present. More branches on lips, tips of fingers. B

Determine the bony walls of the Maxillary sinus: The Anterior wall of the sinus has thin compact bone above the apex of the canine teeth which may extend to the lateral pirifor

Determine Some indicators of Malnutrition? A few of the indicators are enumerated below: 1. Indicators related to Government policies a. Nutrition policy b. Nutrition

#questitypes of plant breeding on..