Nuclear transfer method for gene transfer, Biology

Nuclear transfer method: Nuclear transfer will extend the range of species in which gene targeting will be possible and thereby provide better models to test treatments for human diseases. The production of Dolly followed by subsequent confirmation in cow and mice successfully established a cloning technology, which have opened the new horizons of application of nuclear transfer technique in production of transgenic animals. Current available technique of pronuclear microinjection of a DNA expression cassette is inefficient and expression levels are unpredictable. Nuclear transfer (cloning) offers an appealing alternative. Hence, one could perform gene targeting in cell lines that is able to donate its nuclei to recipient oocytes. This would allow selection of the most productive cell line showing high expression for conversion into highly productive animals, and all animals will be transgenic. For example, the time from microinjection to a milk ­producing flock is 3.5 years, if the founder is female, and 2.5 years, if it is male. With somatic cloning this time can be reduced to 1.5 years. This saving of time will be greater with cow. Production of transgenic founder animals by nuclear transfer also uses less than half the number of experimental animals than a pronuclear injection.

Posted Date: 9/18/2012 7:51:26 AM | Location : United States







Related Discussions:- Nuclear transfer method for gene transfer, Assignment Help, Ask Question on Nuclear transfer method for gene transfer, Get Answer, Expert's Help, Nuclear transfer method for gene transfer Discussions

Write discussion on Nuclear transfer method for gene transfer
Your posts are moderated
Related Questions
Explain Systolic Murmurs in heart diease details? Definition: Murmurs is a series of auditory vibrations that are more prolonged than a sound. Causes: High-energy turbulence ca

Describe the advantages of Clinical neuropsychology Clinical neuropsychology seeks like as understanding, particularly, in the case of how damaged or diseased brain structures

SIMPLE  LIPIDS They are lipids formed of fatty acids and alcohol without any additional group. e.g. fats or true fats,wax,cutin, suberin. Simple lipids are mainly of 2 types

Contrast the reproduction of bacteria with that of frogs. Bacteria replicate asexually by splitting in two. Frogs reproduce sexually by producing sperm and eggs. One sperm and

what is Cytokinesis in cell reproduction? Cytokinesis: Mitosis is usually followed by cytokinesis, the division of the cytoplasm and cell membrane. Cellular organelles are no

Q. Goals of prevention of diabetes mellitus? The goals of prevention are to delay the onset of DM and preventing or delaying the cardiovascular complications. Obesity should be

Septa prevent oxygenated and deoxygenated blood. Give reason

What is absorption?   Absorption involves  the  transfer of materials  through  the mucosa of the  alimentary tract  into blood and lymph vessels.

Explain about the Deranged lipid profile? Lipids, as you are already aware, are important dietary constituents that include fats, steroids, phospholipids and glycolipids. A num

In the early days of microbiology, the disease producing submicroscopic agents were termed as 'filterable viruses' because of their ability to pass through conventional filters whi