Nuclear run-on, Biology

Nuclear run-on is a technique used to estimate the relative rate of the transcription of a given gene, as opposed to steady-state level of the mRNA transcript (which is influenced not just by the transcription rates, but by the stability of the RNA). This method is based on the assumption that the highly-transcribed gene should have more molecules of RNA polymerase bound to it than will the same gene in the less-active state. If it is properly prepared, isolated nuclei will continue to transcribe genes and incorporate 32P into RNA, but only in those transcripts which were in progress at the time the nuclei were isolated. Once the polymerase molecules complete transcript they have in progress, they should not be able to re-initiate the transcription. If that is true, then amount of the radiolabel incorporated into a specific type of mRNA is theoretically proportional to the number of RNA polymerase complexes present on that gene at the time of isolation. A very difficult method, rarely applied appropriately from what I understand

Posted Date: 8/3/2012 6:59:14 AM | Location : United States







Related Discussions:- Nuclear run-on, Assignment Help, Ask Question on Nuclear run-on, Get Answer, Expert's Help, Nuclear run-on Discussions

Write discussion on Nuclear run-on
Your posts are moderated
Related Questions
Leptospirosis Leptospirosis is a general term that denotes all infections of man and animals caused by spirochaetes of the genus Leptospira. At one time, the infection had bee

Explain about the term- Therapeutic Therapeutic approaches that scientists are pursuing include transplanting cells to replace those which are damaged or using growth factors

Q. Can you explain Cholera? Cholera is one of the diseases which had caused epidemics all over the world. The organism which causes the disease is Vibrio cholerae. It is an

Fate Maps The details of the procedure of gastrulation are not simple to understand without the knowledge of positions of the cells of the future germinal layers in the blast

The next step in the nitrogen cycle is the assimilation of inorganic nitrogen in the type of ammonia into organic nitrogen-having compounds. Total organisms assimilate  ammonia  by

Air Pollution - Environmental Pollution The threat due to air pollution became apparent only when some severe episodes caused human casualty in USA, Britain (London) and Japan

I nfectious coryza A highly infectious bacterial disease of chickens caused by Hemop hilus paragallinarum, is characterized by catarrhal inflammation of the upper respirator

How is the nervous system of molluscs organized? Molluscs have well-developed sensory structures. It is established that cephalopods, such as squid and octopus, have eyes with

Brefily describe diffusion ? Diffusion :  Diffusion is the process in which there is a net movement of particles of a substance from an area of higher concentration to an are

Chemical disasters arise from harmful and potentially hazardous chemicals, which if released of misused can pose a threat to the environmental. Its range depends upon military and