Nuclear run-on, Biology

Nuclear run-on is a technique used to estimate the relative rate of the transcription of a given gene, as opposed to steady-state level of the mRNA transcript (which is influenced not just by the transcription rates, but by the stability of the RNA). This method is based on the assumption that the highly-transcribed gene should have more molecules of RNA polymerase bound to it than will the same gene in the less-active state. If it is properly prepared, isolated nuclei will continue to transcribe genes and incorporate 32P into RNA, but only in those transcripts which were in progress at the time the nuclei were isolated. Once the polymerase molecules complete transcript they have in progress, they should not be able to re-initiate the transcription. If that is true, then amount of the radiolabel incorporated into a specific type of mRNA is theoretically proportional to the number of RNA polymerase complexes present on that gene at the time of isolation. A very difficult method, rarely applied appropriately from what I understand

Posted Date: 8/3/2012 6:59:14 AM | Location : United States







Related Discussions:- Nuclear run-on, Assignment Help, Ask Question on Nuclear run-on, Get Answer, Expert's Help, Nuclear run-on Discussions

Write discussion on Nuclear run-on
Your posts are moderated
Related Questions
HUMAN SKELETON - Hard supportive or protective elements of the animal body form the skeleton system. It's study in osteology. EXOSKELETO N - Present outside skin. It

Q. Etiological factor of peptic ulcer? Peptic ulcer results when the neural and hormonal abnormality disrupts the factors that normally maintain mucosal integrity and permit pr

CRP is a marker of systemic inflammation. As the role of inflammation in the initiation and progression of atherosclerosis becomes better understood, CRP has gained prominence as a

The American Heart Association (AHA) has recommended the revised Jones Criteria as a guide for ARF diagnosis. The same have been approved by WHO study group for the diagnosis of in

Can you give me an explanation on the osmoregulation of the Phyla Cnidaria, Ctenophora, Porifera and the Protozoa?


Classification of hydrocolloids by function It must be clear to you now that gum ghatti, gum karaya, gum tragacanth ( classified as exudate gums) locust bean  gum, guar g

sanguivarous mode of feeding in annelida

Effects on Ecosystem - Air pollutants The effects of pollutants on ecosystem become visible after a long period of exposure. In highly industrialised countries the vegetation

What is Class Gastropoda in animal kingdom? Snails and slugs are referred to as gastropods because of their characteristic body plan. "Gastro" is derived from the Greek word fo