Nuclear run-on, Biology

Nuclear run-on is a technique used to estimate the relative rate of the transcription of a given gene, as opposed to steady-state level of the mRNA transcript (which is influenced not just by the transcription rates, but by the stability of the RNA). This method is based on the assumption that the highly-transcribed gene should have more molecules of RNA polymerase bound to it than will the same gene in the less-active state. If it is properly prepared, isolated nuclei will continue to transcribe genes and incorporate 32P into RNA, but only in those transcripts which were in progress at the time the nuclei were isolated. Once the polymerase molecules complete transcript they have in progress, they should not be able to re-initiate the transcription. If that is true, then amount of the radiolabel incorporated into a specific type of mRNA is theoretically proportional to the number of RNA polymerase complexes present on that gene at the time of isolation. A very difficult method, rarely applied appropriately from what I understand

Posted Date: 8/3/2012 6:59:14 AM | Location : United States







Related Discussions:- Nuclear run-on, Assignment Help, Ask Question on Nuclear run-on, Get Answer, Expert's Help, Nuclear run-on Discussions

Write discussion on Nuclear run-on
Your posts are moderated
Related Questions
indicator for testing for the presence of sugar

Q What are the kinds of reproduction that occur in fungi? In fungi there is sexual and asexual reproduction. Fungi reproduce asexually by fragmentation, sporulation and gemmati

TRISACCHARIDES The oligosaccharides are made of three monosaccharide residues. A common trisaccharides is Raffinose which is formed by condensation of galactose-glucose-fr

Beginning with the entry of fatty acid into target cells, describe in detail how inactivation of carnitine can lead to a high level of fatty acids in the blood.

Complications of Exchange Transfusion i) Infection of umbilical vessels and systemic injection related to blood transfusion like malaria, CMV, AIDS and hepatitis B. ii) O

Explain Transposition the Great Arteries with Ventricular Scptal Defect ? Transposition of the great arteries with VSD is an indication for surgery. It is preferable to do VSD

Cyanobacteria carry out photosynthesis using two photosystems as in green plants. Furthermore, other photosynthetic bacteria, like as the purple photosynthetic bacterium Rhodospiri

Q What are the hyphae and the mycelium of pluricellular fungi? The major structures of pluricellular fungi are the hyphae threadlike filaments made of contiguous uni or multinu

Q. Explain Atrial Pressure? The RA pressure wave form has three positive deflections - "a", "c", and "v" waves. The "a" wave is due to atrial systole and follows the P-wave on

Echocardiography is one of the -most frequently used imaging modalities for diagnosing cardiovascular diseases. It i's versatile and is applicable in the entire spectrum of