Non-viral vectors, Biology

Non-viral vectors 

Viral vectors are highly efficient but when it comes to large scale production at the commercial level, non-viral serve as a better choice. These methods produce low immunogenic response and hence are also considered to be safe. With the recent advancement in this area the efficiency of transfection has also improved (Anderson, N.D). The best examples of non viral vectors include:

  • Naked DNA: Plasmid DNA is the best example of naked DNA whose structure is simple and commercial production is relatively cheap. They get easily incorporated into the host genome and do not produce immune response. This naked DNA is directly injected into the desired cells (Gould and Favorov, 2003).

Apart from these few other non viral methods include, the use of DNA complexing reagents, liposomes etc.

Table 1: A table showing comparison of vectors (Gould and Favorov, 2003, Table 1)

Vector

Transgene capacity

Immunogenicity

Genome integration

Long-term expression

Transfer into dividing (D) and quiesent (Q) cells

Plasmid-naked

Unlimited

Low

No

Only in muscle

D and Q

Plasmid-complexed

Unlimited

Low

No

No

D and Q

Ad 1st generation

5 kb

High

No

No

D and Q

Ad 2nd generation

8 kb

High

No

No

D and Q

Ad gutless

37 kb

Less

No

Longer

D and Q

AAV

4 kb

High

Yes and episomal

Yes

D and Q

HSV

35 kb

High

No

Yes

D and Q

Retrovirus

Up to 8 kb

Low

Yes

Yes

D

Lentivirus

Up to 8 kb

Low

Yes

Yes

D and Q

Posted Date: 8/9/2012 6:36:05 AM | Location : United States







Related Discussions:- Non-viral vectors, Assignment Help, Ask Question on Non-viral vectors, Get Answer, Expert's Help, Non-viral vectors Discussions

Write discussion on Non-viral vectors
Your posts are moderated
Related Questions
What is the constitutional unit of proteins? The constitutional units of proteins are the amino acids. Protein Structure Review - a) Image Diversity b) amino acid stru

Explain Ventricular septal defects VSD Chsude Technique ? Ventricular septal defects are closed on cardiopulmonary bypass. Approach is through median stemotomy. Ascending aort

Q Which is the biological molecule that holds the genetic information that is transmitted hereditarily and controls the cellular functioning? The hereditary molecule that contr

What would be the likely immune response ability of an individual with a deletion of a Class II gene of the MHC on one chromosome and a nonsense mutation at the beginning of the sa

Q. What are the events that mark the end and the beginning of the first interphase period? What happens inside the cell in this period? The initial interphase period is the G1.

What is the relation between posterior pituitary gland and diabetes insipidus?

Why are the tropical forests also known as stratified forests? In the tropical forests tall trees of several species have their crowns forming a superior layer under which dive

Counselling refers to professional guidance in resolving personal conflicts and emotional problems. Counselling is a process which enables a person to identify problems and find a

TELEMENTORING Telementoring is used by a nursing teacher providing training/advice? to another nurse consultant at a remote site. The Internet has made teleconsultation and t

1.      Hazard Is An Extreme Event Whereas Disaster Is Its Consequence. 2.      A hazard can occur without human interference but it may become a disaster on account of careless