Non-viral vectors, Biology

Non-viral vectors 

Viral vectors are highly efficient but when it comes to large scale production at the commercial level, non-viral serve as a better choice. These methods produce low immunogenic response and hence are also considered to be safe. With the recent advancement in this area the efficiency of transfection has also improved (Anderson, N.D). The best examples of non viral vectors include:

  • Naked DNA: Plasmid DNA is the best example of naked DNA whose structure is simple and commercial production is relatively cheap. They get easily incorporated into the host genome and do not produce immune response. This naked DNA is directly injected into the desired cells (Gould and Favorov, 2003).

Apart from these few other non viral methods include, the use of DNA complexing reagents, liposomes etc.

Table 1: A table showing comparison of vectors (Gould and Favorov, 2003, Table 1)

Vector

Transgene capacity

Immunogenicity

Genome integration

Long-term expression

Transfer into dividing (D) and quiesent (Q) cells

Plasmid-naked

Unlimited

Low

No

Only in muscle

D and Q

Plasmid-complexed

Unlimited

Low

No

No

D and Q

Ad 1st generation

5 kb

High

No

No

D and Q

Ad 2nd generation

8 kb

High

No

No

D and Q

Ad gutless

37 kb

Less

No

Longer

D and Q

AAV

4 kb

High

Yes and episomal

Yes

D and Q

HSV

35 kb

High

No

Yes

D and Q

Retrovirus

Up to 8 kb

Low

Yes

Yes

D

Lentivirus

Up to 8 kb

Low

Yes

Yes

D and Q

Posted Date: 8/9/2012 6:36:05 AM | Location : United States







Related Discussions:- Non-viral vectors, Assignment Help, Ask Question on Non-viral vectors, Get Answer, Expert's Help, Non-viral vectors Discussions

Write discussion on Non-viral vectors
Your posts are moderated
Related Questions
Class of Subphylum Uniramia - Insecta Class Insecta consists of nearly a million described species. There are more species of insects as compared to all the other species of a

The Family Nutrition Approach This involves relatives/family who  live in the client's household; in assisting  the client to make necessary dietary changes  to  prevent or  to

Define Intermediary Metabolites of an Athletes? Coenzyme Q10 - for the physically active; Co-Q 10 activates cell energy. While you carry out any physical act; run, jump, throw,

Define the Spray Drying Method? Spray drying is a unique drying process since it involves both particle formation and drying. It is most suitable for drying of liquid foods suc

At the present level of the biotechnology what are the main techniques of genetic engineering? The main methods of genetic engineering today are: the recombinant DNA technology

what is .groups of protozoa?

What are the types of changes encountered during the Support phase Adaptation - To accommodate changes to its environment Correction - To uncover defects in the Software

quality is expensive. discuss

PHOSPHORUS It is present in vegetables, grains (oat metal, wheat meal), milk, eggs, cheese, meat, fish, etc. It helps in - (a) Like calcium it is a constituent of bones.

What is the function, other than protection, of the ribs? The ribs help to alter the volume of the thorax during breathing movements.