Non-viral vectors, Biology

Non-viral vectors 

Viral vectors are highly efficient but when it comes to large scale production at the commercial level, non-viral serve as a better choice. These methods produce low immunogenic response and hence are also considered to be safe. With the recent advancement in this area the efficiency of transfection has also improved (Anderson, N.D). The best examples of non viral vectors include:

  • Naked DNA: Plasmid DNA is the best example of naked DNA whose structure is simple and commercial production is relatively cheap. They get easily incorporated into the host genome and do not produce immune response. This naked DNA is directly injected into the desired cells (Gould and Favorov, 2003).

Apart from these few other non viral methods include, the use of DNA complexing reagents, liposomes etc.

Table 1: A table showing comparison of vectors (Gould and Favorov, 2003, Table 1)

Vector

Transgene capacity

Immunogenicity

Genome integration

Long-term expression

Transfer into dividing (D) and quiesent (Q) cells

Plasmid-naked

Unlimited

Low

No

Only in muscle

D and Q

Plasmid-complexed

Unlimited

Low

No

No

D and Q

Ad 1st generation

5 kb

High

No

No

D and Q

Ad 2nd generation

8 kb

High

No

No

D and Q

Ad gutless

37 kb

Less

No

Longer

D and Q

AAV

4 kb

High

Yes and episomal

Yes

D and Q

HSV

35 kb

High

No

Yes

D and Q

Retrovirus

Up to 8 kb

Low

Yes

Yes

D

Lentivirus

Up to 8 kb

Low

Yes

Yes

D and Q

Posted Date: 8/9/2012 6:36:05 AM | Location : United States







Related Discussions:- Non-viral vectors, Assignment Help, Ask Question on Non-viral vectors, Get Answer, Expert's Help, Non-viral vectors Discussions

Write discussion on Non-viral vectors
Your posts are moderated
Related Questions
Effects of long term malnutrition on adults In adults, once the stored carbohydrates and fats are used up, the body starts using proteins to generate energy. These protei

X-Linked Dominant Genes Dominant X-linked genes are always expressed in both the sexes just as in autosomal traits. One dose of X-linked dominant allele produces its effects in

What are the symptoms and foods associated with campylobacteriosis?  Campylobacteriosis incubation period: 2-5 days after ingestion of contaminated food.  Symptoms: Diarrhoe

a) Where casparian strips situated in a plant body are and what are they made up of? Mention its function. b) What is respiration quotient (RQ)? Under what conditions will th

Q. What Treatment should be used for cardiac tamponade? Percutaneous Pericardiocentesis Cardiac tamponade is an acute emergency and percutaneous pericardiocentesis must b

What is Ground Meristem in primary growing shoot? The ground meristem produces new cells which ultimately develop into parenchyma cells. The central portion of the primary shoo

Motion Sickness  The pathogenesis of motion sickness is poorly under- stood. The prescription cholinergic blocker scopolamine in a patch or oral formulation can decrease sympto

Speculate on the necessity of interdependence of volvox organisms in the colonial existence

What are main limitations on size that can be synthesized and how will that be change over next few years? A: Today, a 10kb gene or longer is built as part of the regular produ

Problem 1: Briefly describe the different types of parenteral routes of drug administration. Definition of parenteral routes of administration. Show different types of