Methods of gene transfer in transgenic animals, Biology

Methods of gene transfer

The technology employed for introduction of transgenes into a livestock population may produce consequences specific to the method. Once introduced, transgenic animals will become subject to the traditional selection criteria of livestock producers and the market forces for the gene products involved. A transgene will likely be expanded into the breeding population so long as the associated trait remains desirable in production systems and the marketplace. By use of marker-assisted selection, a few transgenes could be selectively incorporated into or eliminated from an otherwise diverse breeding population without great difficulty. The method resulting in Polly is seen as a major improvement as compared to the technology of the early nineties that led to the first transgenic bovine creature, carrying the human lactoferrin gene (lactoferrin is an important component in breast milk). At that time, genes were injected into newly fertilized eggs, and only in rather infrequent cases did the gene stably integrate and lead to a transformed animal.

Although transgenic mice were produced three decade ago, the mouse is still the animal of choice and used as model system for most of the experiment.  However, currently pigs are used for most experiments because of its potential transgenic frequency.  Following are some of the gene transfer methods carried out in animals.

  1. Retroviral method
  2. Microinjection Method
  3. Nuclear transfer method

 

Posted Date: 9/18/2012 7:49:13 AM | Location : United States







Related Discussions:- Methods of gene transfer in transgenic animals, Assignment Help, Ask Question on Methods of gene transfer in transgenic animals, Get Answer, Expert's Help, Methods of gene transfer in transgenic animals Discussions

Write discussion on Methods of gene transfer in transgenic animals
Your posts are moderated
Related Questions
Explain the periodic table of the elements ? Periodic Table of the Elements :  An element is a pure substance composed of atoms all of a single kind. It cannot be broken down

Why is it important for core body temperature to stay more constant than skin temperature?

Q. What is the relation between secretion of parathormone and the calcium blood level? The parathormone increases the calcium blood level since it stimulates the resorption (re

Define Regulation of Water Balance - Hormonal Control of Fluid Balance? Hormonal control of fluid balance: When water intake is insufficient or water loss is excessive, the kid

Q. Medical therapy for tricuspid stenosis? Medical therapy is ineffective. With diuretics, symptoms of congestion are replaced by that of low cardiac output state. Occasional c

Determine Causes of Malnutrition? You may recall studying about the causes of malnutrition earlier. The causes of malnutrition are classified as immediate (individual level), u

TEST TUBE BABY - Ova are fertilized in laboratory dish (in vitra) containing a nutrient broth . It may be artificial insemination donor (AID) / Artificial insemination h

How many of the same proteins are made at the same time by each ribosome in the translation of one mRNA molecule? How does consecutive protein production occur in translation?

α - amylase activity by GA 3 - Dormancy Of the enzymes required for the digestion of starch α-amylase appears immediately after the start of germination. It was found that

You have 200 cells and find 120 in interphase, 57 in prophase, 8 in prometaphase, and 5 in metaphase, 8 in anaphase and 2 in telophase. (None in G0 phase). it takes 18 hours to com