Method to estimate phytochrome, Biology

Method to Estimate Phytochrome

Since there is no chemical test, the only method available to test and estimate phytochrome is the spectrophotometric method. Firstly, the difference in the absorbance between 660 and 730 nm is measured after red light irradiation (Δ OD red, No. 1.). Next, the sample is irradiated with far-red light and again the difference in Δ OD is measured (Δ OD far-red, No. 2). The content of total phytochrome is measured by subtracting the value obtained after red light irradiation from far-red light irradiated value.

2343_Method to Estimate Phytochrome.png

Nowadays special, spectrophotometers are also available which can measure the absorption difference between two wavelengths. These are called dual wavelength spectrophotometers.

Posted Date: 1/17/2013 7:45:08 AM | Location : United States







Related Discussions:- Method to estimate phytochrome, Assignment Help, Ask Question on Method to estimate phytochrome, Get Answer, Expert's Help, Method to estimate phytochrome Discussions

Write discussion on Method to estimate phytochrome
Your posts are moderated
Related Questions
What are the main human degenerative diseases? The main human degenerative diseases are separated into three groups: cardiovascular diseases, neoplastic diseases and degenerat

Explain the Transmission Electron Microscope Here, thin and dehydrated specimen is used. Electrons pass through the specimen and form image on to photographic film. These are u

What are the final digestion products of (a) protein, (b) fat, (c) starch?   a) Proteins are digested to amino acids, b) fats are digested to fatty acids and glycerol,

Explain transposition with VSD with restricted pulmonary? Transposition of great arteries with VSD with restricted pulmonary blood flow: Restriction to pulmonary blood flow

How to use microorganisms to control diseases

When water containing impurities is forced against a filter that allows only water through, this process is called "reverse osmosis". Why is this a good description of what is happ

different applications of microbiology

Determine the Food Source for Phosphorus? Phosphorus is widely distributed in food. Food phosphorus is a mixture of both organic and inorganic forms although the relative amoun

Name the four organisms which are currently in danger of extinction because of human activities?

Proteolytic enzymes They catalyze the hydrolysis of peptide bonds in other proteins. In order to prevent them doing generalized damage to all cellular proteins, they are often