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The first step of the purification of an integral membrane protein is to disrupt its interactions with other integral proteins and the lipids in the membrane. This is commonly achieved by adding a detergent that solubilizes the membrane. In the order to solubilize membrane but not denature the protein mild detergents like as octyl glucoside or Triton X-100 is used rather than the harsh detergent SDS. they readily intercalate into the lipid disrupt & bilayer the hydrophobic interactions as the detergent molecules are themselves amphipathic .
figure: Structures of (a) Triton X-100 and (b) octyl glucoside.
figure: (a) Detergent solubilization and (b) reconstitution into artificial lipid vesicles of an integral membrane protein.
Q. In which meiotic division does the separation of the homologous take place and what are the ploidies of the generated cells after the end of that process? The separation of
Explain Development of excretory system? The filtration rate of kidney is low and infants find it difficult to eliminate high concentration of solutes. However, by the end of t
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Define interaction of Vitamin C with Riboflavin? The two coenzyme forms of riboflavin are FMN and FAD. They are found in a large number of systems which function in the metabol
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Hybridoma is the clone of plasmacytoma cells which secrete a monoclonal antibody; commonly produced by the fusion of peripheral or splenic plasma cells taken from the immunized mo
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Describe the tailored to the individual patient? Patent ductus arteriosus (PDA) interruption (surgical or transcatheter intervention): It is a curative procedure. Atrial
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