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You may have heard in the news currently that a new species of frog was discovered within a rainforest.
You objective are to isolate genomic DNA and construct an expression library by using λ phage. You are interested within cloning the gene which encodes an enzyme that produces an intense green skin color and also you have an antibody which you think will work.
Questions:
a) Design a well controlled experiment to illustrate that your antibody will work for the library screening. Sketch a picture of your expected experimental effects as part of your reply.
b) Propose two reasonable explanations why you may not be capable to isolate the DNA even if you plated three genomes worth of DNA into your screening.
Define Sample Titration - estimation of vitamin c in chillies? Take a clean and dry large chilli and weigh it. Note the weight of the chilli. Grind the chilli to a fine paste i
Problem 1: Briefly describe the different types of parenteral routes of drug administration. Definition of parenteral routes of administration. Show different types of
Members belonging to the scientific community fear the misuse of this therapy leading to dangerous consequences. People may try to insert the desired gene, for example, the gene f
A dominant allele will always increase in frequency over time. Yes No Q44. When a gene has two alleles, the allele frequencies in a population automatically move toward an equilibr
To study the conditions essential for the germination of seeds:- In the diagram below a having seeds on cotton wool with air, warmth, but no water; b has water, warmth, but no
What is a species? A species is a set of living beings able to cross between themselves generating fertile offspring. This concept though does not apply to individuals of ex
what are intergenic interactions ? please explain with example of cross breeding. and how we calculate the phenotype and genotype. explain epistasis, duplicate genes, supplementary
How calcium prevents luxury consumption of potassium Increasing Ca:K ratio in soil solution depresses the uptake of potassium. Thus one of the harmful effects of over liming m
Bond specificity Some enzymes act on a particular bond (glycosidic, peptide, ester etc.). Examples: pepsin, trypsin, chymotrypsin etc. are all only acting on
Are there ribosomes in a plant cell? Yes, in fact there are ribosomes in both plant and animal cells. That is right. Ribosomes are the site of synthesis of polypeptide chain
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