Inverse pcr, Biology

Inverse PCR is the variation of PCR which makes the amplification of DNA segments of unknown sequence that flank DNA segments of the known sequence possible; in short, total DNA is digested to completion and fragments ligated under conditions that favour circularization of the fragments; pair of the PCR primers, designed from known sequence, are used to prime PCR from the opposite strands resulting in amplification of fragment of unknown sequence.

Posted Date: 8/3/2012 7:04:16 AM | Location : United States







Related Discussions:- Inverse pcr, Assignment Help, Ask Question on Inverse pcr, Get Answer, Expert's Help, Inverse pcr Discussions

Write discussion on Inverse pcr
Your posts are moderated
Related Questions

he Lac Repressor protein, which we will talk about a bit later in the course, is important for controlling expression of a number of genes in E. coli. There are about 10 copies of

Pathophysiology   The exact mechanism of glomerulonephritis  is still under investigation. The dies'ease is characterised by deposition  of immune complexes  consisting of anti

What is cardio vasular examination in hheart disease? Cardio-vascular examination: A thorough and systematically cardio-vascular examination provides valuable clues to the pr

Q. Acid Hydrolysis of Non-Reducing Sugar? It involves the acid hydrolysis of non-reducing sugars to reducing sugars. It is also known as the process of inversion. Acid hydrolys

What do all organic compounds contain? The key element is carbon. Organic compounds are all carbon-having compounds. By definition, an organic compound having carbon. Alt

Flowering and Endogenous Rhythms The last four decades of studies in animals and plant physiology have clearly established the existence of a biological clock mechanism to me

The gene is a distinct portion of a cell's DNA. Genes are coded instructions for making all the body needs, mainly proteins. Human beings have about 25,000 genes. Researchers have

Describe how a phagocyte destroys bacteria. The phagocyte forms a pouch in its cell membrane and engulfs bacteria in the pouch. It then pinches off the pouch to produce a vesi

Considering a pair of homologous chromosomes containing a gene having two different alleles how many different genotypes can the individual present? If a gene of the diploid sp