Inverse pcr, Biology

Inverse PCR is the variation of PCR which makes the amplification of DNA segments of unknown sequence that flank DNA segments of the known sequence possible; in short, total DNA is digested to completion and fragments ligated under conditions that favour circularization of the fragments; pair of the PCR primers, designed from known sequence, are used to prime PCR from the opposite strands resulting in amplification of fragment of unknown sequence.

Posted Date: 8/3/2012 7:04:16 AM | Location : United States







Related Discussions:- Inverse pcr, Assignment Help, Ask Question on Inverse pcr, Get Answer, Expert's Help, Inverse pcr Discussions

Write discussion on Inverse pcr
Your posts are moderated
Related Questions
Q. List the counselling skills? Slowly start winding up the contact with the client and the family by providing them support, motivating the client to take care of oneself with

What is vena cava? Which type of blood circulates within the vena cava? The vena cava are either of two large veins that debouch into the right atrium. The superior vena cava d

In designing an experiment to find out whether light is required for photosynthesis    (a) what is the principle of the design    (b) what control would you use?

Q. Show Clinical symptoms of galactosemia? Clinical symptoms of galactosemia deficiency is found to appear early in infancy. Some infants are born with cataract, jaundice and

Wind Strong current of air is known as wind, it is an important ecological factor as it affects plant life mainly on flat plains, along sea coasts and at high altitudes in moun

In a test cross: A.alleles of two genes is always involved. B.One parent must be homozygous recessive C.the physical manifestation of a trait can be deterimined. D.both A and C are

Illustrate about the Ward Halstead and Luria The linear approach is best exemplified in the work of A.R. Luria and various collaborators, while the configurational approach is

Determine the purposes of vital signs The vital signs reflect the functioning of vital organs of the body i.e. heart, brain and lungs. They indicate the basic functions of body

Explain the soft diet The soft diet provides soft whole food that is lightly seasoned and moderately low in fibre. The foods have a soft  texture and are easy  to digest. Smal

Explain the Irradiation and its Implication The issues are the decreased salivary flow, liability for infection because of the decreased blood supply and the possibility of ost