Illustrate about sterilization protocol, Biology

Q. Illustrate about Sterilization protocol?

Sterilization protocol includes proper transportation and cleaning of instruments followed by their packing. Items can be sterilized using modalities like dry heat such as flaming, or moist heat like pasteurization, boiling though highly unreliable as a sterilization technique and more effective for disinfection, it is used very often.  Autoclave means self locking and is used to denote an apparatus that sterilizes by use of steam under pressure. It operates on same principle as pressure cookers. Radiation is cold sterilization with no heat involved; non ionising radiation include infrared and UV rays. Lethal effect is through generation of heat and DNA damage. Penetration is low but efficacy is high. By chemicals such as alcohols: ethyl alcohol, isopropyl alcohol; aldehydes: formaldehyde: widely employed for the fumigation of operation theatres. Gutaraldehyde: commercial preparations are active at different pH and have percentage of 2-3.2. They can destroy microbes in 10 hours. Gases: ethylene oxide. The choice of opting for a particular mode of sterilization depends on the physical and chemical properties of the items to be sterilized. Proper sterilization needs to be coupled with aseptic storage envoirnment.

Second part of infection control includes operatory room protocol which starts with preparation of operatory followed by preparation of patient and the operator. For the operator  use of personal protective equipment (PPE) - clothing, masks, eyewear, and gloves and Hand washing is considered important measures to reduce the risk of transmitting organisms to patients and HCWs (health care workers). The purpose of washing is twofold: to reduce the both transient and resident flora for the duration of a procedure to protect the operator in the event that there is non-intact skin on the hand, thereby accomplishing the aim of maintaining the "chain of asepsis".

Posted Date: 7/27/2013 5:53:54 AM | Location : United States







Related Discussions:- Illustrate about sterilization protocol, Assignment Help, Ask Question on Illustrate about sterilization protocol, Get Answer, Expert's Help, Illustrate about sterilization protocol Discussions

Write discussion on Illustrate about sterilization protocol
Your posts are moderated
Related Questions
Discuss the biological importance of Imidazole. Bring in examples of biomolecules that contain this group. Please be as thorough as possible.

How is snow - blindness caused in humans? a) Mention the site where syngamy happens in amphibians and reptiles correspondingly.

Q. What is the difference between diastoleSystole and systole and diastole are the two stages into which the cardiac cycle is divided Diastole is the stage of the cardiac cycle

What is the swinging flashlight test used for It is done to check the relative afferent pupillary defect in an eye that is blind from optic nerve disease.

The condition in which there is a DECREASE in the number of white blood cells in humans is termed as: a) Leukocytosis (pron: lew-kO-sigh-toe-sis) b) Leukopenia (pron: lew-kO

Kidney - 2 in number. Dark red. Slightly flattened. Bean shaped. 10 cm. long, 5 cm wide and 4 cm thick (In rabbit 20-25 mm). Weighing 150 gm in male 135 gm in fema

Are sex-linked diseases associated only to genes of the X chromosome? There are many X-linked diseases, like hemophilia A, hemophilia B and adrenoleukodystrophy, but called as

Explain Systemic Circulation in human biology? In systemic circulation, oxygenated blood is pumped from the left atrium through the bicuspid valve, into the left ventricle, and

Facilitation Model - Models of Succession This is considered as the classical model of succession. It is based on the assumption that species of a previous stage are replaced

Q What are the two major classes into which fishes are divided? Ans. Fishes are divided into two major classes: osteichthyes, bony fishes (tuna, sardines, salmons) , and chondr