Illustrate about sterilization protocol, Biology

Q. Illustrate about Sterilization protocol?

Sterilization protocol includes proper transportation and cleaning of instruments followed by their packing. Items can be sterilized using modalities like dry heat such as flaming, or moist heat like pasteurization, boiling though highly unreliable as a sterilization technique and more effective for disinfection, it is used very often.  Autoclave means self locking and is used to denote an apparatus that sterilizes by use of steam under pressure. It operates on same principle as pressure cookers. Radiation is cold sterilization with no heat involved; non ionising radiation include infrared and UV rays. Lethal effect is through generation of heat and DNA damage. Penetration is low but efficacy is high. By chemicals such as alcohols: ethyl alcohol, isopropyl alcohol; aldehydes: formaldehyde: widely employed for the fumigation of operation theatres. Gutaraldehyde: commercial preparations are active at different pH and have percentage of 2-3.2. They can destroy microbes in 10 hours. Gases: ethylene oxide. The choice of opting for a particular mode of sterilization depends on the physical and chemical properties of the items to be sterilized. Proper sterilization needs to be coupled with aseptic storage envoirnment.

Second part of infection control includes operatory room protocol which starts with preparation of operatory followed by preparation of patient and the operator. For the operator  use of personal protective equipment (PPE) - clothing, masks, eyewear, and gloves and Hand washing is considered important measures to reduce the risk of transmitting organisms to patients and HCWs (health care workers). The purpose of washing is twofold: to reduce the both transient and resident flora for the duration of a procedure to protect the operator in the event that there is non-intact skin on the hand, thereby accomplishing the aim of maintaining the "chain of asepsis".

Posted Date: 7/27/2013 5:53:54 AM | Location : United States







Related Discussions:- Illustrate about sterilization protocol, Assignment Help, Ask Question on Illustrate about sterilization protocol, Get Answer, Expert's Help, Illustrate about sterilization protocol Discussions

Write discussion on Illustrate about sterilization protocol
Your posts are moderated
Related Questions
Define Dietary Management of Sepsis with or without MODS? Before we begin with the dietary management of patients suffering from sepsis with or without MODS let us read a case

Weiss's Theory of Fibroosseous Fixation Weiss' theory stated that there is a fibro-osseous ligament formed between the implant and the bone and this ligament can be considered

Define Water as a temperature regulator? Water plays an important role in the distribution of heat throughout the body and the regulation of body temperature. Heat is generate

Q. Explain about gamma diversity? Gamma (g) richness refers to the rate of change across larger landscape gradients and gamma diversity is a measure of the overall diversity fo

Q. Including major events and hormonal changes how can the menstrual cycle be described? One can imagine a cycle like an analog clock at which at 0 o'clock is the beginning and

Q. How Linnaeus classify the plant kingdom? Linnaeus classified the plant kingdom into 24 classes in his famous work 'Genera Plantarum' (1737) and 'Species Plantarum' (1753). I

Beta-blockers have traditionally been considered contraindicated in patients with heart failure because they may block the compensatory actions of the sympathetic nervous system wi

Formation of cocoon in earthworm: In earthworm segments from 14 to 17 are swollen. Skin on these segments is thick and glandular. This is called clitellum. When ovaries ma

Explain Feeding Schedule in Infancy? 1) Milk feed 5-6 times/day. Continue breast-feeding. Exclusive breast-feeding up to 6 months even during illness. Follow demand feeding.

different types of animals and their respiratory organ