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How successful are you at synthesizing genes with really high G/C content?
A: We have an extremely low failure rate, which is about one out of five thousand or ten thousand genes, most probably because of toxicity of a gene and not because of high GC content. High GC constructs indeed are more demanding but nevertheless part of the routine production, based on a special method, which takes one week longer but is very reliable.
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a) A recombinant DNA is formed when sticky ends of vector DNA and foreign DNA join. b) Define how the sticky ends are formed and get joined.
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Integral plasma membrane proteins are also synthesized by ribosomes on the RER, but become inserted in the rough endoplasmic reticulum membrane rather than transported into the lum
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