How much template dna will you add, Biology

You are setting up a PCR reaction. a) The total volume of your reaction is 50 uL. Your stock primer solutions are 25 mM each. You want a final concentration of primer of 1 mM in your reaction. How much primer do you need to add? b) For the same reaction in (a), you have a stock concentration of template DNA that is 10 ug/mL. You want to add a total of 50 ng to your reaction. How much template DNA (volume) will you add

Posted Date: 2/28/2014 4:32:15 AM | Location : United States

Related Discussions:- How much template dna will you add, Assignment Help, Ask Question on How much template dna will you add, Get Answer, Expert's Help, How much template dna will you add Discussions

Write discussion on How much template dna will you add
Your posts are moderated
Related Questions
How is plant tissues classified according to their functions? Plant tissues are separated into growth (embryonic) supporting, tissues, filling and photosynthetic tissues (groun

Are sperms being attacked by the body since they are foreign?

In sulfur photosynthetic bacteria what is the molecule that donates hydrogen for photosynthesis? In sulfur photosynthetic bacteria the substance that donates hydrogen is hydrog

Response to Wounding Wounding of tomato plants by mechanical injury or chewing by insects releases a factor that triggers accumulation of two proteinase inhibitors-throughout

Define Requirements for underwater weighing method? • The equipment required to perform hydrostatic measurements is bulky and maintenance intense. • A large tank of water, usu

What gases will be taken in and given out by a green plant (a) in darkness, (b) in bright sunlight?   a) In low light intensities a green plant will be taking in oxygen and

Nerves - Organisation of Nervous System Due to the pattern of distribution of neurons as described above, there are bundles of nerve fibers, called nerves, connecting the cent

P o x diseases Small-pox in human beings and pox in a few animal species are closely related to each other. It is shown by the fact that the vaccine for the prevention of sma

Unit Membrane Model Lamellar model was supported by Robertson. According to Robertson - All biomembrane have similar structure (like PM), on the basis of this observati