How much template dna will you add, Biology

Assignment Help:

You are setting up a PCR reaction. a) The total volume of your reaction is 50 uL. Your stock primer solutions are 25 mM each. You want a final concentration of primer of 1 mM in your reaction. How much primer do you need to add? b) For the same reaction in (a), you have a stock concentration of template DNA that is 10 ug/mL. You want to add a total of 50 ng to your reaction. How much template DNA (volume) will you add?


Related Discussions:- How much template dna will you add

Explain the stool weight and laxation, Explain the Stool weight and laxatio...

Explain the Stool weight and laxation? The amount of stool excreted varies markedly from individual to individual and in an individual over a period of time. Faeces are complex

Vitamin - k, VITAMIN - K Also known as naptho quionone / anti haemo...

VITAMIN - K Also known as naptho quionone / anti haemorrhagic vitamin / coagulation vitamin / menadone . It requires for formation of blood clotting factor prothrombin.

Phylum mollusca, what is the correct classification of phylum mollasca

what is the correct classification of phylum mollasca

Compare and contrast the effect of a deletion, Compare and contrast the eff...

Compare and contrast the effect of a deletion in the operator of the lactose operon with one in the operator of the histidine operon.

What is the function of the citric acid cycle, What is the function of the ...

What is the function of the citric acid cycle? The hnction ofthe  citric acid cycle can be discussed as  follows. The  intermediates of citric acid cycle are used as  precursor

Explain what is biomes and their types, Explain what is Biomes and their ty...

Explain what is Biomes and their types? Scientists study biological systems as units. For instance, an ecosystem unit consists of a community and its physical environment. The

Nutrition, how do carnivorous feed

how do carnivorous feed

Common eye defects, COMMO N EYE DEFECTS - 1 .       Glaucoma - Pres...

COMMO N EYE DEFECTS - 1 .       Glaucoma - Pressure of aquous humour is increased, retina is degenerated. 2 .       Presbyopia - Due to non etastic cilliary muscles,

Cloning protocol, Sally Subcloner wants to clone a fragment into pUC19 (Map...

Sally Subcloner wants to clone a fragment into pUC19 (Map above – From New England Biolabs). Her fragment was a protein coding gene of 2.7 kb, and had XhoI sites on either end (and

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd