Genomic dna fragments, Biology

You have an extensive library of genomic DNA fragments, prepared by the use of restriction nucleases. You know their sequences and sizes and you have an assay to reliably separate and identify them by electrophoresis on agarose gels. It has been reported that a newly isolated protein forms tight complexes with some of the DNA fragments.

If you can identify those DNA fragments, you might be able make a very educated "guess" as to the identity of the gene that is regulated by the protein. You do not yet have the sequence and structural information about the protein, so bioinformatics approaches are not going to be especially useful. How would you proceed? Be clear as to what type of assay(s)) you would do, and specifically how you could use the protein, and an antibody to the protein to identify candidate DNA fragments/genes.

 

Posted Date: 3/4/2013 8:17:34 AM | Location : United States







Related Discussions:- Genomic dna fragments, Assignment Help, Ask Question on Genomic dna fragments, Get Answer, Expert's Help, Genomic dna fragments Discussions

Write discussion on Genomic dna fragments
Your posts are moderated
Related Questions
Auxins The history of discovery of auxin is a fascinating chapter in plant physiology. The hormone auxin was discovered first, through some elegant experiments by Charles Darw

Archaeological data provide valuable information about the uses of plants in the past. Information comes from a number of sources, including fossil plant and human remains as well

Define Functions of Calcium in Human Body? Calcium salts provide rigidity to the skeleton and calcium ions play a role in many if not most, metabolic processes. In the vertebra

The hydrolysis of the disaccharide lactose (in milk) give up glucose and galactose. Thus galactose is also a major dietary sugar for humans. Glucose and Galactose are epimers which

Q. What is the etiological agent of amebiasis? How it transmitted and what are the typical manifestations of the disease? The Amebiasis is caused by the protozoan Entamoeba his

Define Factors that affect the requirement of Protein? Protein requirement is greatly influenced by many factors such as age, environmental temperature, energy intake, gender,

Classify biomaterials and list some examples. Biologic classification is based on tissue response and systemic toxicity effects of the implant and is divided into three classes


Q. What are introns, and how do they affect the processing of genetic information? Introns, a feature of eukaryotic DNA, are intervening stretches of DNA lying between the exon

Importance of reproduction Reproduction is an important life process in animals and plants. Reproduction is the ability of an organism to produce new generation of indivi