Genomic dna fragments, Biology

You have an extensive library of genomic DNA fragments, prepared by the use of restriction nucleases. You know their sequences and sizes and you have an assay to reliably separate and identify them by electrophoresis on agarose gels. It has been reported that a newly isolated protein forms tight complexes with some of the DNA fragments.

If you can identify those DNA fragments, you might be able make a very educated "guess" as to the identity of the gene that is regulated by the protein. You do not yet have the sequence and structural information about the protein, so bioinformatics approaches are not going to be especially useful. How would you proceed? Be clear as to what type of assay(s)) you would do, and specifically how you could use the protein, and an antibody to the protein to identify candidate DNA fragments/genes.

 

Posted Date: 3/4/2013 8:17:34 AM | Location : United States







Related Discussions:- Genomic dna fragments, Assignment Help, Ask Question on Genomic dna fragments, Get Answer, Expert's Help, Genomic dna fragments Discussions

Write discussion on Genomic dna fragments
Your posts are moderated
Related Questions
What is Machined implant Machined implant has irregular surface roughness from approximately 10μm grooves and ridges in the machining direction. The roughness of implants can

What is Temperate Grasslands? Grasslands are known by their common names in different regions of the world, such as the plains or prairies in North America, the steppe in Asia,

Describe the Classification of Life in diversity of life? There are at least 10 million different kinds of living organisms inhabiting Earth. Many different organisms have comm

What are the advancement of cnidaria over protozoa

Unprecedental clarity has come to our understanding of genetic differences by the analysis of DNA sequences. It is not surprising in which the latest DNA technologies are leading t

How boron affect the the synthesis of proteins ? Inadequate availability of boron tends to increase the loss of phosphorus from plant roots and also affects the synthesis of pro

ADAP T A TIO N - Characters that make an organism to adjust well and suited to its way of life. Variation occur in living organism due to change in environment. Th

Adverse effects of Fungal Prophylaxis - Micafungin is generally well tolerated. Adverse events have included fever, headache, nausea, vomiting, diar-rhea, leukopenia, hepatic e

How is claimant need to make balance of probability? When there is no loss suffered through a person after that there cannot be a civil proceeding not like under criminal law t

what are the excretory organs of birds