General protocol for media preparation, Biology

General Protocol for Media Preparation

A general protocol for media preparation is as follows:

1. Prepare stock solutions one day before the medium is to be made.

2. Weigh the required quantities of agar and sucrose and dissolve them in water (about 314th the final volume of the medium) by heating in a-water bath or autoclaving at low pressure.

3. Pipette the required volumes of each of the stock solutions into the above solution kept on a stirrer.

4. Make up the final volume of the medium by addition of distilled water.

5. Adjust the pH to 5.8 with 0.1-0.5 N NaoH or Hcl.

Posted Date: 1/24/2013 4:50:36 AM | Location : United States







Related Discussions:- General protocol for media preparation, Assignment Help, Ask Question on General protocol for media preparation, Get Answer, Expert's Help, General protocol for media preparation Discussions

Write discussion on General protocol for media preparation
Your posts are moderated
Related Questions
characters of phylum protozoa

Increase in cardio-thoracic ratio is a relatively specific indicator of left ventricular end-diastolic volume. Left atrial enlargement is seen as double density shadow, lifting up

extracellular signals and their characteristics

A field trip to observe flowers:- Plan a field trip to view flowers in bloom. If no interesting wildflowers can be found growing near the school, the trip can be planned to a p

Q. Define X-Ray Chest and Coronary Angiography? X-Ray Chest It shows cardiomegaly with left ventricle (LV) and left atrium (LA) enlargement. There may be signs of pulmona

general cherecter and anatomical stucture

Intracellular sodium concentration of the motor neuron A complete motor neuron is removed from a frog and placed in normal physiological saline at 1 AM.  The neuron is healthy.

This process involves the regulation of the concentration of the body fluids through control of water content and salt content of the blood. If a person loses water from their bloo

Photosynthesis is a process used by plants and other organisms to change the light energy captured from the sun into chemical energy which can be beneficial to fuel the organism's

mentions chareacteristics and classificaton of protozoa?