Gene targeting, Biology

Gene targeting: There is always a debate on gene integration in to the host animal.

It is random gene integration Vs gene targeting. Perhaps most significantly, pro-nuclear injection cannot be used to modify the animals' own genes. By contrast, target modifications to endogenous genes have been achieved earlier in mouse model and now in farm animals also. When introduced into the early embryo these cells contribute to the germline. They can also undergo homologous recombination with transfected foreign DNA so that precise, targeted changes can be introduced. The combination of these two properties means that gene targeting of the mouse germline is now routine, allowing for the deletion, replacement or modification of selected genes. Recombination on either side of the marker gene results in its precise insertion into the chromosome at the same time and delete some or the entire target gene. Precise targeting events are very rare (perhaps 1 in 106 cells) and, therefore special methods must be taken for identifying and selecting the correctly targeted cells.

Posted Date: 9/18/2012 7:51:47 AM | Location : United States







Related Discussions:- Gene targeting, Assignment Help, Ask Question on Gene targeting, Get Answer, Expert's Help, Gene targeting Discussions

Write discussion on Gene targeting
Your posts are moderated
Related Questions
Clone (verb) is the action of duplicating the genetic material within a vector. To clone the piece of DNA, one would insert it into some type of the vector (like, a plasmid) and p

I have a test online it is timed 30 minutes on Anatomy and Physiology I need an expert who is an expert in this subject to take it for me

Which of the following most accurately explains the meaning of a repression lag? A. A repression lag refers to the time that it takes for the last full-length mRNA transcript t

Q. What are biopolymers? Polymers are macromolecules made by the union of several smaller identical molecules, called monomers. Biopolymers are polymers present in the living b

Toxoplasma cycles between its rodent and feline hosts, living out different phases of its existence in each. What is the scientific means about the cat and mouse?

The citric acid cycle also called as the TCA cycle (tricarboxylic acid cycle) and the Krebs cycle or the Szent-Györgyi-Krebs cycle is a series of chemical reactions used by all aer

structural adaptation of mammalian alimentary canal

Q. Show the Physical criteria of quality for Honey? Colour, crystallization, pH, acidity, water content are some of the criteria used for analysis of honey. These criteria are

Q. Introduction of Quality Attributes of Food? Seasonal fruits and vegetables are grown in plenty, and are available in their respective seasons in abundance. While choosing th

Migration - Population Dispersal Dispersal is affected by the presence or absence of the barrier and vagility which means inherent power of movement also called dispersal powe