Gene targeting, Biology

Gene targeting: There is always a debate on gene integration in to the host animal.

It is random gene integration Vs gene targeting. Perhaps most significantly, pro-nuclear injection cannot be used to modify the animals' own genes. By contrast, target modifications to endogenous genes have been achieved earlier in mouse model and now in farm animals also. When introduced into the early embryo these cells contribute to the germline. They can also undergo homologous recombination with transfected foreign DNA so that precise, targeted changes can be introduced. The combination of these two properties means that gene targeting of the mouse germline is now routine, allowing for the deletion, replacement or modification of selected genes. Recombination on either side of the marker gene results in its precise insertion into the chromosome at the same time and delete some or the entire target gene. Precise targeting events are very rare (perhaps 1 in 106 cells) and, therefore special methods must be taken for identifying and selecting the correctly targeted cells.

Posted Date: 9/18/2012 7:51:47 AM | Location : United States







Related Discussions:- Gene targeting, Assignment Help, Ask Question on Gene targeting, Get Answer, Expert's Help, Gene targeting Discussions

Write discussion on Gene targeting
Your posts are moderated
Related Questions
What is the difference between osmosis and diffusion? Osmosis is the occurrence of movement of solvent particles (in general, water) from a region of lower solute concentration

Using the words 'cilia' and 'mucus', describe, very briefly, how the body gets rid of dust which enters the lungs. The lining of the air passages makes mucus which traps dust p

Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4

How do plants solve the problem of transporting substances throughout their tissues? In bryophytes the substance transport is completed by diffusion. In tracheophytes (pteridop

Creb binding protein (CBP) is a histone acetyl transferase (HAT) protein. What role does theĀ  addition of acetyl groups to histones play during development? A. The addition of

Geometric isomers: Select one: a. Have the same molecular weight b. Have the same connectivity c. Have a different configuration about a double bond d. All of the above

Materials Handling and Storage Material handling is a very significant process in working place. The materials are handled in not finished, semi finished and finished states.

Q. What are the difference between plasma membrane and cell wall? Plasma membrane and cell wall is not the same thing. Plasma membrane, also known as cell membrane, is the exte

Q. Show Nutrition guidelines for prevention of heart disease? Nutrition guidelines for prevention of heart disease as suggested by WHO are highlighted. Details regardin

What are phospholipids? Phospholipids are molecules made of glycerol bound to two long molecules of fatty acids and to one phosphate group. Thus, phospholipids are amphipathic