Gene targeting, Biology

Gene targeting: There is always a debate on gene integration in to the host animal.

It is random gene integration Vs gene targeting. Perhaps most significantly, pro-nuclear injection cannot be used to modify the animals' own genes. By contrast, target modifications to endogenous genes have been achieved earlier in mouse model and now in farm animals also. When introduced into the early embryo these cells contribute to the germline. They can also undergo homologous recombination with transfected foreign DNA so that precise, targeted changes can be introduced. The combination of these two properties means that gene targeting of the mouse germline is now routine, allowing for the deletion, replacement or modification of selected genes. Recombination on either side of the marker gene results in its precise insertion into the chromosome at the same time and delete some or the entire target gene. Precise targeting events are very rare (perhaps 1 in 106 cells) and, therefore special methods must be taken for identifying and selecting the correctly targeted cells.

Posted Date: 9/18/2012 7:51:47 AM | Location : United States







Related Discussions:- Gene targeting, Assignment Help, Ask Question on Gene targeting, Get Answer, Expert's Help, Gene targeting Discussions

Write discussion on Gene targeting
Your posts are moderated
Related Questions
Summary of the Phylum and protozoa and the important

Q. Carbohydrates requirement during diabetes? Carbohydrates: These to a large extent depend on the food habits. Complex carbohydrates with more fibre are recommended to simple

do fats with one or more 'kinky' tail fatty acids tend to be solid or liquid at room temperature? These are found in triglycerides forming what? Solid fats or oils? Is it opposite

Leptospirosis Leptospirosis is a general term that denotes all infections of man and animals caused by spirochaetes of the genus Leptospira. At one time, the infection had bee

Etiology: This is the study of origin of diseases. In other words we can say etiology is the study of causation and origination. Etiology most commonly used in philosophical theori

Determine the food sources of riboflavin? The food sources of riboflavin include: 1. Rich sources: Liver, dried yeast, egg powder, milk powder. 2. Good sources: Who

Q. Illustrate Physiological Evidence ? Physiological and biochemical evidence is providing data of increasing importance to plant systematic. Recently, it has become apparent t

How Infections and Infestations cause PEM? Childhood infections (viral/bacterial) and parasitic infestations are almost always associated with PEM. These cause anorexia (loss o

Signify the term - Analogy, Analogous? Refers to structures that do not have the same evolutionary origin but have the same functions. The wings of a bat and insect are analogo

Nucleotides Compounds  consisting of a  nitrogenous  base,  a  pentose sugar and a phosphate group.