Freeze fracture electron microscopy, Biology

Freeze Fracture Electron Microscopy

Freeze fracture electron microscopy i a technique lo reveal the surface features of hydrophobic interior of cell membrane. In this technique, the cells/tissues are first frozen in liquid freon at - 179°C. The frozen block is cut with a cold knife blade alongwith the planes of weakness, run through each cell. Generally, these planes occur between the two layers of lipid molecules of the membrane. The plane of fracture at these places exposes the interior of a plasma membrane (Figure shown below).

1017_freeze microscopy.jpg


The newly exposed faces of a plasma membrane, the exoplasrnic fracture face (EF) and the protoplasmic fracture face (PF) are covered with a very thin layer of platinum by dissolving away the organic material. The freshly fractured membrane may be covered with platinum and carbon to dtain replicas (exact copies) of the surface and this is observed under a TEM. This technique demonstrates that intercellular junctions (where cells make contact with each other) are composed of specilaised membrane protein.

Posted Date: 12/10/2012 1:01:34 AM | Location : United States







Related Discussions:- Freeze fracture electron microscopy, Assignment Help, Ask Question on Freeze fracture electron microscopy, Get Answer, Expert's Help, Freeze fracture electron microscopy Discussions

Write discussion on Freeze fracture electron microscopy
Your posts are moderated
Related Questions
Explain what happens when the cell membrane or plasma membrane ruptures or breaks down? Ans) When cell membrane ruptures Ions leek out and unless repaired in time the cell will

Residue is defined medically as the solid contents that have reached the lower intestine. A low residue diet is composed of foods, which are easily digested and readily absorbed, r

The energy - releasing metabolic process in which substrate is oxidised without an external electron acceptor is known as: 1. Glycolysis 2. Fermentation 3. Aerobic respira

Q. what is Catabolism? Catabolism is a process of breaking down large or complex molecules of food components into smaller components. These supply energy to the cell in a form

Determine the Emerging clinical use of brain Emerging clinical uses include identifying brain regions from which epileptic seizures emanate and confirming diagnoses of neurodeg

what SOAP is: Subjective - The data includes information obtained from the patient or the patient's family regarding the problem. Objective dat a - the data is gathered  f

Cheese Production Cheese production involves the conversion of the milk protein, k-casein to paracasein by a defined, limited hydrolysis, catalysed by chymosin (rennin). In the

What is the importance of the uterine glycogen-producing glands? The uterine glands produce glycogen that can be degraded into glucose to nourish the embryo before the complete

What would happen to the energy flow through an ecosystem if the decomposers were eliminated?  Without decomposers, the nutrients kept in the bodies and wastes of other organis

Q. Can you explain Tannins? Tannins are another class of compounds which interfere with the absorption of minerals like iron and reduce the availability of proteins by bindin