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Freeze Fracture Electron MicroscopyFreeze fracture electron microscopy i a technique lo reveal the surface features of hydrophobic interior of cell membrane. In this technique, the cells/tissues are first frozen in liquid freon at - 179°C. The frozen block is cut with a cold knife blade alongwith the planes of weakness, run through each cell. Generally, these planes occur between the two layers of lipid molecules of the membrane. The plane of fracture at these places exposes the interior of a plasma membrane (Figure shown below).
The newly exposed faces of a plasma membrane, the exoplasrnic fracture face (EF) and the protoplasmic fracture face (PF) are covered with a very thin layer of platinum by dissolving away the organic material. The freshly fractured membrane may be covered with platinum and carbon to dtain replicas (exact copies) of the surface and this is observed under a TEM. This technique demonstrates that intercellular junctions (where cells make contact with each other) are composed of specilaised membrane protein.
In humans, the recessive allele that causes a form of red-green colour blindness (c) is found on the X chromosome. a) Determine the genotypes and phenotypes of the F1 generation fr
Functionality of implant material It should take maximum advantage of available bone and permit the maximum amount of forces to be transmitted through the implant within physio
Prevention You as nurse needs to ensure that iron supplementation is given to children who are at risk for iron deficiency. Iron supplementation should start between 4 and
Treatment and Nursing Management The initial treatment is surgical repair of the cleft. The timing of the repair varies from surgeon to surgeon, but most prefer cleft lip rep
Define the Causes of Failures of Root Canal Treatment 1. Errors in diagnosis and treatment planning 2. Coronal leakage ( inadequate restorative protection) 3. Lack of kno
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Q. What does radial symmetry means? What is the kind of symmetry found in chordates? Which are other phyla of the animal kingdom that present species with radial symmetry? Radi
A post-operative client has an order for Demerol 35 mg and Phenergan 12.5 mg. The nurse reviews the labels of the medications. Injectable Demerol (meperidine hydrochloride) is prep
Which Foods allowed in soft diet:- Soups - mildly flavoured - broths and cream soups. Beverages - all Meat - moist, tender meat, fish or chicken, cottage cheese, eggs
Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not
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