Food-borne clostridium, Biology

Food-borne Clostridium


Clostridium botulinum and Cl. perfringens, the two members of the group have been implicated in food-borne illnesses. C. botulinum causes classical food-borne botulism due to ingestion of preformed toxin in food.


Epidemiology:
Clostridia grow anaerobically in rewarmed food dishes produce toxin. These are gram-positive, anaerobic sporulating, catalase negative rod shaped organisms.


Clinical features:
In case of poisoning due to Cl. botulinum, incubation period var ies from 24-9 6 ho urs. Toxin absorbe d fro m gut blo cks acetylcho line at neuromuscular junction. Diplopia, dysphagia, dysphonia and respiratory involvement occur. Mortality rate is high.Incubation period in case of Cl. perfringens ranges from 8-16 hours. Enterotoxin produced during sporulation in gut causes hyper secretion. Sudden onset of profuse diarrhoea occurs that may occasionally be accompanied with vomiting. Recovery takes in 1- 4 days.


Diagnosis:
Robertson cooked meat medium is used for enrichment, however, other specific enrichment media are also available. The horse blood agar and anaerobic egg yolk agar are the recommended plating media for isolation of Cl. botulinum. The colonies are raised or flat, smooth or rough and commonly show some spreading with irregular edge. The selective media for isolation of Cl. Perfringens are tryptose sulphate cycloserine (TCS) agar, sulphate-polymyxin-sulphadiazine (SPS) agar, Shahid Ferguson perfringens (SFP) agar and neomycin blood agar, etc. PCR based detection of Cl. perfringens targeting alpha gene or epsilon toxin has been used.

Posted Date: 9/20/2012 2:49:22 AM | Location : United States







Related Discussions:- Food-borne clostridium, Assignment Help, Ask Question on Food-borne clostridium, Get Answer, Expert's Help, Food-borne clostridium Discussions

Write discussion on Food-borne clostridium
Your posts are moderated
Related Questions
List host factor which play important role in implant survival. Host factors: a) Medical status of the patient b) Habits - Smoking - Parafunctional habits c) O

Foot as Burrowing Organ The Scaphopoda that usually have burrowing habit, possess foot suitably modified for burrowing purpose. The conical and protrusible foot of Dentalium

Explain Procedure for Performing the Completed Coliform Test? Completed test is the last step in the Coliform test procedure which is described herewith. Conduct the exercise f

F ALLOPIAN TUBE - Fertilisation occur in it. 10-12 cm long muscular tube. It is supported by double fold of peritonium. It shows 4 regions - (i ) Infundibulum - Bro

THEOR Y OF GERMPLASM - August Weismann (1834-1914) criticized the inheritance of acquired characters by putting forward the theory of continuity of germplasm. According

Explain the Classification of Malignant Tumors Malignant tumors can be classified as: Carcinomas: These tumors arise from the epithelial lining (the squamous cells) e.g. ski

Explain for Procedure Quantitative Determination of Viable Microbes? Now carry out the exercise following the steps included herewith: 1. Label the diluent tubes from 10 -1

Specify the term in detail - Swim bladder. Found in bony fish, this gas-filled chamber is used to main neutral buoyancy. Oxygen in blood is added or removed as required. Swim b

Non Symbiotic Nitrogen Fixers - Nutrient Cycles There are certain groups of free living bacteria both aerobic and anaerobic and blue green algae that fix nitrogen. Aerobic nit

Define requirements of Iodine during pregnancy period? You would be already aware that maternal iodine deficiency leads to cretinism in the off spring. Hence, the material diet