Fluorescence microscopy, Biology

Fluorescence Microscopy

Certain compounds when exposed to short wavelength radiations, e.g., UV and X-rays. absorb and emit energy as light of a longer wavelength. This process of emitting light from a compound is known as fluorescence and is now commonly used in microscopy. Compounds which naturally emit light exist in most living tissues. For example, the mitochondria of liver and kidney, collagen and chlorophyll give a strong fluorescence. Such a type of natural fluorescence is called as autofluorescence. Sometimes fluorescent dyes llKe rhodamine, quinacrine etc. called fluorochromes are used to stain cell components to produce secondary fluorescence. Such important dyes are also used in the study of chromosome behaviour.

The fluorescent microscope is essentially an ordinary optical instrument. It has been modified by the incorporation of special filters. These filters allow only the required wavelength of light to pass and cause fluorescence in the specimen. The most significant application of fluorescent microscopy is in the field of immuno-fluorescent,antibody labelling technique about which you will study later in this Unit.

Posted Date: 12/10/2012 12:37:20 AM | Location : United States







Related Discussions:- Fluorescence microscopy, Assignment Help, Ask Question on Fluorescence microscopy, Get Answer, Expert's Help, Fluorescence microscopy Discussions

Write discussion on Fluorescence microscopy
Your posts are moderated
Related Questions
Secretion of Hormones The secretion of most hormones (except steroid) is by the process of exocytosis. Figure summarises the formation, transport, release and reconstitution o

Coronary Vasoconstriction 1) Coronary flow limiting stenoses are caused by concentric or eccentric artherosclerotic plaques, with or without potential for local vasomotor change

Work Injuries Based On Type of Accidents Earlier we identified four kinds of injuries mainly based upon type of disability. This classification is needed for the purposes of c

Q. What is ST Heart Rate Index? To calculate the index, the maximum ST depression is divided by the Delta heart rate (difference between the resting and maximum heart rate). As

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Q. Explain about Oral Glucose Tolerance Test? This is most commonly used diagnostic test particularly for identifying new and ‘at risk' individua1s.Thi.s test is carried out af

#what is naturalistic theory?

Why is it important for core body temperature to stay more constant than skin temperature?

Carbon Based Materials These include Pyrolytic carbon, polycrystalline (Vitreous Carbon), and carbon/silicon interstitial combination. Vitreous Carbon -Solid or porous vitre

Clotting Disorder - Haemophilia Haemophilia  is a congenital  blood clotting  disorder caused by the genetic lack/ deficiency of  coagulation factor VIII or antihaemophiliac