Fluorescence microscopy, Biology

Fluorescence Microscopy

Certain compounds when exposed to short wavelength radiations, e.g., UV and X-rays. absorb and emit energy as light of a longer wavelength. This process of emitting light from a compound is known as fluorescence and is now commonly used in microscopy. Compounds which naturally emit light exist in most living tissues. For example, the mitochondria of liver and kidney, collagen and chlorophyll give a strong fluorescence. Such a type of natural fluorescence is called as autofluorescence. Sometimes fluorescent dyes llKe rhodamine, quinacrine etc. called fluorochromes are used to stain cell components to produce secondary fluorescence. Such important dyes are also used in the study of chromosome behaviour.

The fluorescent microscope is essentially an ordinary optical instrument. It has been modified by the incorporation of special filters. These filters allow only the required wavelength of light to pass and cause fluorescence in the specimen. The most significant application of fluorescent microscopy is in the field of immuno-fluorescent,antibody labelling technique about which you will study later in this Unit.

Posted Date: 12/10/2012 12:37:20 AM | Location : United States







Related Discussions:- Fluorescence microscopy, Assignment Help, Ask Question on Fluorescence microscopy, Get Answer, Expert's Help, Fluorescence microscopy Discussions

Write discussion on Fluorescence microscopy
Your posts are moderated
Related Questions

2 phases of oogenesis

Explain Kashin Beck disease caused by selenium deficiency? Kashin Beck disease was identified to affect growing children in parts of Siberian Russia and China. It is characteri

What are the chemical elements that form most of living biological matter? The elements are:- a)  oxygen (O), b)  carbon (C), c)  hydrogen (H) and d) Nitrogen (N).

Define Food Effects in the Survival & Growth of Microorganism? Nature of the food also affects the microbial survival and growth. Each food has its own characteristics which de

The classification was given by Spaulding and is based on the contact with different tissue types to determine whether sterilization or disinfection is needed. 1. Critical item

Q. What is xaxim? Most pteridophytes have subterraneous stems similar to the substrate called as rhizomes. Xaxim is a kind of pteridophyte with an aerial stem in generally perp

Population You must be familiar with the term 'population'. It is one of the most talked about issues of this century. It is feared that the rapid growth of the world population

Q. What is the second polar body? After termination of the second meiotic division of the oocyte II two cells are generated: the second polar body and the egg cell proper. The

Define Future challenges of management of renewable resources?   In response to past shortcomings in a few fisheries management, and to dealing with other conservation goals