Explain transfer of culture from one liquid media to another, Biology

Assignment Help:

Explain Transfer of Culture from One Liquid Media to another Liquid Media?

1. Inoculating loop is to be held in the right hand. It is sterilized by incineration in the inner blue portion (hottest) of the flame until it becomes red hot. Upper steel portion of the handle is also passed rapidly through the flame. The loop is cooled by holding for 10-20 seconds in air near the flame or by dipping in fresh medium. Never place the sterilized loop on working bench.

2. The broth culture tube is thoroughly mixed by thumping the bottom of the tube. Both the culture tube and the fresh broth tube are grasped in left hand.

3. Cotton plugs or caps of both the tubes are removed by holding one cap with the little finger and the second with the next finger of right hand (Hand carrying the inoculating loop). The caps should be kept in the hand with the inner aspect of the caps or plugs pointing away from the palm of the hand. Never place caps or cotton plugs on the working bench to avoid contamination.

4. The mouth of both the tubes is passed through the flame and then the loopful of the culture is transferred from the culture tube to fresh medium.

5. Again the mouth is flamed to avoid contamination and the caps/plugs are replaced to respective tubes.

6. Inoculating wire is reflamed immediately after transfer, to destroy adhering microorganisms.

7. The tubes are incubated in the incubator or shaker at 37C overnight.

 


Related Discussions:- Explain transfer of culture from one liquid media to another

What is rate of photosynthesis in c3 plants, What would happen to the rate ...

What would happen to the rate of photosynthesis in C3 plants if the CO2 concentration level almost doubles from its present level in the atmosphere?

What happens to the dna molecule when the ends, Now try to pull the strands...

Now try to pull the strands apart at the "origin of replication." What happens to the DNA molecule when the ends are restricted

Explain the procedure for morphological study of fungi, Explain the Procedu...

Explain the Procedure for Morphological Study of Fungi? Now carry out the exercise, following the steps enumerated herewith: 1. Prepare a humid chamber by placing two-three

Nature and structure of community, Nature and Structure of Community E...

Nature and Structure of Community Every place on earth -grasslands, forests, and ponds, edge of the river or sea is shared by many coexisting organisms. Plants, animals and mi

What is plastids, What is Plastids? The most obvious difference between...

What is Plastids? The most obvious difference between plant cells and other eukaryotic cells is that cells of most plants contain unique organelles called plastids, which inclu

What is polysaccharides, What is Polysaccharides? Polysaccharides:  M...

What is Polysaccharides? Polysaccharides:  Many monosaccharide subunits can be joined to form long, complex polysaccharide molecules. These complex sugars are often used to s

Define the bioavailability of vitamins, Define the Bioavailability of Vitam...

Define the Bioavailability of Vitamins? The term bio availability refers to the overall efficiency of utilization, including physiological and biochemical processes involved in

Explain phyium oomycetes, Phyium Oomycetes 1) They reproduced asexually...

Phyium Oomycetes 1) They reproduced asexually by non-motile conidia and/or mobile flagellatedzoospores. 2) The sexual reproduction is by fusion of a male gamete with an egg

Seed germination - development of plant, Seed Germination - Development of ...

Seed Germination - Development of plant The environmental factors that influence the germination of seeds are: Water availability, Optimal light, Aeratio

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd