Explain three parts of homogenate, Biology

Assignment Help:

Differential centrifugation divides cellular components based on the differences in their size and / or density. A tissue sample was homogenized and three parts (aliquots) of this homogenate were subjected to differential centrifugation using three dissimilar methods as shown below:

Method I: Aliquot 1 was centrifuged at 200,000g for 2 h.

Method II: Aliquot 2 was centrifuged at dissimilar speeds and for variable durations as shown below:

Step 1: 1,000g for 10 min

Step 2: 20,000g for 20 min

Step 3: 80,000g for 1 h

Step 4: 200,000g for 2 h

Method III: Aliquot 3 was processed in the same manner as aliquot 2, except that the sediment and supernatant were divided after each step.

 Cellular components can be obtained as separate fractions from which of these methods?

a.  Only method I 

b.  Only method II

c.  Only method III 

d.  Both methods II and III

 


Related Discussions:- Explain three parts of homogenate

Various definitions of species, In biology, a species is smallest and basic...

In biology, a species is smallest and basic unit of classification. Each species is placed within a single genus. For example biological name of human being is homosapiens. Here ho

Anomalies related to oral cavity, Anomalies Related to Oral Cavity   Un...

Anomalies Related to Oral Cavity   Under these anomalies we will briefly discuss the cleft lip and cleft palate.  You must have seen and/or nursed a baby born with a cut on

Dissolved gases and alkalinity, Dissolved Gases and Alkalinity Disso...

Dissolved Gases and Alkalinity Dissolved Gases The marine environment serves as a gigantic reservoir of dissolved oxygen and carbon dioxide, which respectively help reg

Locomotion in leech, LOCOMOTION IN LEECH - Leech moves by looping actio...

LOCOMOTION IN LEECH - Leech moves by looping action of its body on substratum. Posterior sucker is attached to base, body elongates, then anterior sucker is attached. Now po

Explain food applications of dextran, Food Applications Dextran can be ...

Food Applications Dextran can be used in food products, as it is capable of moisture retention and inhibition of crystallization of sugar. The properties of dextran as gelling

Nitrogen control of nitrogen assimilation, Nitrogen Control of Nitrogen Ass...

Nitrogen Control of Nitrogen Assimilation N 2 -fixer like Klebsiella pneumoniae and Nostoc can grow with N 2 , NO - 3 or NH + 4 as nitrogen source. You would like to know

What are the two divisions of the angiosperms, What are the two divisions o...

What are the two divisions of the angiosperms? The angiosperms are separated into monocotyledonous and dicotyledonous. (These categories are defined later in this text.) Pla

State problem of hypoglycaemia when driving, Hypoglycaemia When Driving ...

Hypoglycaemia When Driving Hypoglycemia is particularly dangerous if it happens to someone who is driving. People with hypoglycemia may have trouble concentrating or se

What are the two main subdivisions of the phylum Chordata, Normal 0 ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4 What are the two mai

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd