Explain the determination of vitamin A, Biology

Assignment Help:

Determination of Vitamin A

Vitamin A assay is carried out by chemical method. This method is called Carr Price Reaction. 

 Vitamin A and the carotenoids produce an intense blue colour upon addition of a saturated solution of antimony trichloride in anhydrous and alcohol-free chloroform. This reaction is called Carr Price Reaction and used to determine Vitamin A.  The absorption maxima is around 620 nm.

 In another method, absorption of vitamin A dissolved in isopropyl alcohol is determined at wavelengths of 310, 325 and 334 nm. This method is suitable for the determination of pure vitamin A (or of the ester after their saponification), such as oily solutions as well as in tablets, coated tablets, capsules and other formulations.

 


Related Discussions:- Explain the determination of vitamin A

Science class, Im learning about difference cell structures in science. I w...

Im learning about difference cell structures in science. I was wondering if I might be able to get some information about the epithelial tissue, and the connective tissue. Thankyou

Pasteur''s experiment, Louis Pasteur (1860-1862) French): Even after th...

Louis Pasteur (1860-1862) French): Even after the experiments of Spallanzani, the conflict of a biogenesis and biogenesis continued well into the middle of 19 th century till

Medicinal value - conservation of wildlife, Medicinal Value - Conservation ...

Medicinal Value - Conservation of Wildlife About 40% of all drugs used throughout the world have active ingredients extracted from plants and animals. The world-wide annual sa

What are nerve function, The major function of the nerve cells of the body ...

The major function of the nerve cells of the body is contact. This is accomplished by passing electrical and chemical messages from neuron to neuron or from neuron to one other goa

Nitrogen fixation and assimilation, Nitrogen is needed mostly for the synth...

Nitrogen is needed mostly for the synthesis of amino acids and nucleotides. Sources of nitrogen can be obtain from organic as well as inorganic sources, but the whole target is the

Describe valvar aortic stenosis murmurs, Describe Valvar Aortic Stenosis Mu...

Describe Valvar Aortic Stenosis Murmurs ? Characteristic: Harsh, rasping crescendo-decrescendo murmur best heard anywhere in a straight line from right 2nd ICS to apex ("sash"

Glucose, Glucose is a six-carbon single sugar; It is the most common energ...

Glucose is a six-carbon single sugar; It is the most common energy source.

Circuitry and priming -procedures of oxygenators, Circuitry and Priming : ...

Circuitry and Priming : The cardio pulmonary bypass circuit consists of oxygenator, tubings, cannulae, cardiotomy reservoirs and cardioplegia attachments. The first step is to pri

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd