Explain restricted fragment polymorphism, Biology

Q. Explain Restricted fragment polymorphism?

This method makes use of a group of enzymes termed restriction enzymes which recognise particular sequence of DNA and act as "molecular scissors" to cut at the beginning and end of these sequences. When DNA from one individual is cut this way, and the resulting fragments are subjected to electrophoresis, the fragments become aligned by length enabling the recognition of a pattern. The analysis of patterns from different individuals permits an assessment of variation in their DNA sequences. Measuring the differing frequencies of the various patterns in a population or species can indicate the genetic diversity within the group.

Posted Date: 8/22/2013 6:21:24 AM | Location : United States

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