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Explain Precautions for Isolation of Pure Culture?
1. Plating of the medium should be done 24 hours before performing the streaking.
There should not be any moisture on the plate.
2. Petri plate lid should not be lifted completely.
3. Labeling of the plate should be done at the bottom of the plate.
4. Flaming and reflaming of the loop should be done to get the desired separation of the organism.
5. Before using the inoculating loop, cooling should be done by touching the agar surface away from the streaks, otherwise the organisms would be killed.
6. Maintain aseptic conditions.
7. Avoid pressing the loop too firmly against the agar surface as this will damage it.
8. Never let the loop to touch any of the previously streaked ones.
control of microorganisms by chemical method.
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organism that can not exist in the presence of oxygen ?
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steps involved in glycolysis
Ram Ventilation Some fish do not use pumping action for gill ventilation. It has been known for long that large tunas cannot be kept alive in captivity unless they are put in
The PCR (polymerase chain reaction) is an extremely simple as immensely powerful methods. It permits enormous amplification of any particular sequence of DNA
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