Explain how blue white selection works, Biology

The plasmids below show the insertion of the EZH2 gene into the pBluescript plasmid in either sense (forward) or antisense (reverse) orientation. These are the plasmids you will hopefully have at the end of your cloning practical. NB - C is the cloning enzyme you used (i.e. EcoRI) and HindIII (indicated as B on the image) is the enzyme you will use to test for inserts.

2462_Explain how blue-white selection works.png

a) Draw a gel image with the pattern of bands you would expect to see after digesting with the 'forward' or 'reverse' plasmids with HindIII 

b) List the exact sizes of the fragments for each of the digests in a) 

c) Insertion of EZH2 into the pBluescript plasmid can be selected for on the basis of blue-white selection. Explain how blue-white selection works.

Posted Date: 3/25/2013 5:10:52 AM | Location : United States







Related Discussions:- Explain how blue white selection works, Assignment Help, Ask Question on Explain how blue white selection works, Get Answer, Expert's Help, Explain how blue white selection works Discussions

Write discussion on Explain how blue white selection works
Your posts are moderated
Related Questions
Difference between Sympathetic and Parasympathetic Nervous System - S.NO . SYM P A THETIC PERASYM P A THETIC 1.

Transmission electron microscopy: Around 1931-32 two German scientists. Knoll and Ruska. Invented transmission electron microscopy, and built the first transmission electron m

You have identified a protein in a cell that you know that comes from the human body. With respect to that protein you have also determined that it allows sodium ions to go through

List three human activities which could cause the loss of a species. Human activities which threaten species with extinction are: (i) hunting of individual species, (ii) over-

#questioen..what is the example of outline og phylum coelenterata

Inportant step for gene clonning

Q. Complications during rheumatic heart disease? Inflammation of Inning of heart (pericarditis), anaemia, heart ,enlargement, valve deformities (mitral and tricuspid valves),

This innovative, pacemaker-based approach to the treatment of patients with heart failure who have a wide QRS complex (>140 ms) on 12-lead ECG aims at providing electromechanical c

Define the Primary Stain and Mordant? (i) Primary Stain - Crystal violet is the primary or first stain, which stains all the cells violet/purple. (ii) Mordant - Gram's iodin

There are three levels of diversity viz. genetic, species and ecosystem diversity. In effect, these levels cannot be separated. Each is important, interacting with a nd influencin