The plasmids below show the insertion of the EZH2 gene into the pBluescript plasmid in either sense (forward) or antisense (reverse) orientation. These are the plasmids you will hopefully have at the end of your cloning practical. NB - C is the cloning enzyme you used (i.e. EcoRI) and HindIII (indicated as B on the image) is the enzyme you will use to test for inserts.

a) Draw a gel image with the pattern of bands you would expect to see after digesting with the 'forward' or 'reverse' plasmids with HindIII 

b) List the exact sizes of the fragments for each of the digests in a) 

c) Insertion of EZH2 into the pBluescript plasmid can be selected for on the basis of blue-white selection. Explain how blue-white selection works.

Posted Date: 3/25/2013 5:10:52 AM | Location : United States

Ask an Expert

Related Discussions:- Explain how blue white selection works, Assignment Help, Ask Question on Explain how blue white selection works, Get Answer, Expert's Help, Explain how blue white selection works Discussions

Write discussion on Explain how blue white selection works
Your posts are moderated

Write your message here..

Related Questions

Protozoans, Protozoans Prokaryotes represent only a few thousand of th... Protozoans Prokaryotes represent only a few thousand of the estimated 2 million species of living beings known on the earth today. The rest are mostly eukaryotes that form the

Explain classification of lipids, Classification of lipids  A general c... Classification of lipids  A general classification of lipids based on their structural components is presented in table. Such a classification, however, is possibly too rigid f

Factors causing short-term changes in intra-ocular pressure, What Factors c... What Factors causing short-term changes in intra-ocular pressure 1) Arterial blood pressure 2) Systemic venous pressure (SVP) 3) Mechanical pressure 4) Plasma osmolari

Technique-recurrence of angina, Technique:  Careful re-opening of ste... Technique:  Careful re-opening of sternum and release of steinum from the underlying adhesion is done. Femoral artery and vein are exposed for going on femoro fenioral bypass

Uses of drugs, USES OF DRUGS - 1.       Drugs are prescribed as medicin... USES OF DRUGS - 1.       Drugs are prescribed as medicines to prevent or cure a variety of diseases. 2.       May be used to promot physical and mental health. 3.       U

Does thermal inversion occur in the winter or in the summer, Does thermal i... Does thermal inversion occur in the winter or in the summer? Pollutant low altitude thermal inversion happens in the winter. In this period of the year the sun heats the soil l

Oxygenator- equipment of cardio pulmonary bypass , Oxygenator :   They serv... Oxygenator :   They serve the function of lungs during extra corporeal circulation (ECC) - Oxygenation, removal of carbon dioxide and transport of gaseous anaesthetic agents:*The o

Calcium - mineral elements, CALCIUM It is the most abundant mineral of... CALCIUM It is the most abundant mineral of animal body. Calcium is available in all types of vegetables, grain, milk, cheese, eggs, fish and butter. By its deficiency r

Translation in prokaryotes, Term Translation or protein synthesis, is quite... Term Translation or protein synthesis, is quite similar in eukaryotes and prokaryotes. We will look at the details of the process in prokaryotes and consider the differences in euk

Describe the examination of abdomen, Describe the Examination of Abdomen ? ... Describe the Examination of Abdomen ? The contour of abdomen is to be recorded. Normally the abdomen is scaphold in sharp in a young adult. In case of fullness and bulge presen