The origin of true life , eubiont from a pmtobiont involves the acquisition of three major features:
1) assembly of phospholipids and proteins into a cell membrane forms the boundary of the cell,
2) development of protein-directed metabolic pathways to utilize the organic molecules for energy needs, and
3) formation of nucleic acid molecules that are not only capable of self-replication but are also capable of storing genetic information for the synthesis of proteins. Since we have no definite idea of the smcture of a protobiont, it is difficult to speculate the manner in which a eubiont evolved from a protobiont. All the same, it is certain that a eubiont comparable to the simplest living cell today must have originated first.
It is quite likely that the formation of a boundary membrane would have taken place before the acquisition of a genetic information system and metabolic pathways. A separation of the cell interior from the outside, would permit sufficient interaction of molecules inside the cell.