Ethidium bromide, Biology

Ethidium Bromide intercalates within structure of the nucleic acids in such a manner that they fluoresce under the UV light. Ethidium bromide staining is generally used to visualize RNA or DNA in the agarose gels placed on the UV light boxes. Proper precautions are needed, because the ethidium bromide is extremely mutagenic and the UV light damaging to eyes. Ethidium bromide is also involved in cesium chloride gradients during the process of ultracentrifugation, to separate the supercoiled circular DNA from linear and the relaxed circular DNA.

Posted Date: 8/3/2012 6:47:03 AM | Location : United States

Related Discussions:- Ethidium bromide, Assignment Help, Ask Question on Ethidium bromide, Get Answer, Expert's Help, Ethidium bromide Discussions

Write discussion on Ethidium bromide
Your posts are moderated
Related Questions

Theoretical Foundation of Halsted Reitan battery There are really two theoretical bases for the Halsted Reitan battery, one contained in brain and intelligence and related writ

Blotting method to detect particular one proteins Ans) Western blotting

Non-threshold  approaches For  genetic carcinogenes, the  "NOEL-safety factor"  approach  is generally  not considered a suitable method for setting the acceptable intake level

Define the elements that affects the Fitness? Fitness varies from a person to person. It is influenced by age, sex, heredity, personal habits, exercise and eating practices. Th

Explain Deficiency diseases due to vitamin C? Vitamin C deficiency in human's results in the disease called scurvy, whose symptoms include hemorrhaging (especially in the gums)

Q. Show types of hiatus hernia? Depending on the extent of herniation and placement of stomach, it could be of two types: 1, Para Oesophageal Hernia (oesophagus is in normal

Define Food Intake and Energy Requirements for Humans? Many studies have shown that the subjects loose significant amounts of body mass; fat mass, as well as, fat free mass du

Question 1 List the differential staining techniques used in microbiology laboratory. Explain Gram staining technique in detail. Add a note on modifications of Gram staining and a