Ethidium bromide, Biology

Ethidium Bromide intercalates within structure of the nucleic acids in such a manner that they fluoresce under the UV light. Ethidium bromide staining is generally used to visualize RNA or DNA in the agarose gels placed on the UV light boxes. Proper precautions are needed, because the ethidium bromide is extremely mutagenic and the UV light damaging to eyes. Ethidium bromide is also involved in cesium chloride gradients during the process of ultracentrifugation, to separate the supercoiled circular DNA from linear and the relaxed circular DNA.

Posted Date: 8/3/2012 6:47:03 AM | Location : United States







Related Discussions:- Ethidium bromide, Assignment Help, Ask Question on Ethidium bromide, Get Answer, Expert's Help, Ethidium bromide Discussions

Write discussion on Ethidium bromide
Your posts are moderated
Related Questions
Chlorine (Cl) - Micronutrients The role of Cl - in plant is not clearly understood. Cl - is required in Hill reaction, the water splitting reaction of photosystem II. In the

What are the cells produced in the first stage of the embryonic development called? The cells that result from the cleavage (the first stage of the embryonic development) are k

Importance of ecology

Using an average wavelength of 450 nm (0.45 um) can you resolve two objects that sit greater than 200nm apart from one another? Use mathematical calculations to justify your answer

Ecosystem as a Unit of Nature An ecosystem can be visualised as a functional unit of nature representing complex interactions between living and non-living components. Th

What is tree girdling? What happens to a plant when that girdle is removed from the stem (below the branches)? Malpighi's girdling, or tree girdling, is the removal from a ste

Bilateral Cleavage In some animals (for example tunicates and nematodes, although in different way), the arrangement of 4 blastomeres after second cleavage is almost radially

Kinds of Ovules Ovules undergo varied degrees of curvature during development. The position of micropyle with respect to the funiculus becomes different and constitutes the b

Explain Research dietitians Research dietitians work in the field of normal or therapeutic nutrition Research dietitians seek ways to improve the nutrition of both  healthy

Q. What are the animal pole and the vegetal pole of the vertebrate egg? The animal pole of a telolecithal egg is the portion of the egg with little vitellus it is opposite to t