Establishment of suspension cultures, Biology

Establishment of Suspension Cultures

A callus crumbles into smaller clumps and single cells in liquid medium by gentle agitation (100-120 rPM) on a shaker. Shaking the cultures also helps to aerate the P - cells. Such suspension cultures however rarely comprise single cells alone because cells tend to aggregate in clusters of 2-100. Suspension cultures can be maintained indefinitely by inoculations of known aliquot5 of cells to a fresh medium. This process is termed as "batch cultures". Alternatively, the medium is replenished at regular intervals. This process is termed as "continuous culture". In the continuous culture process at the time of replenishing the medium, cells are also harvested (open continuous system) or the biomass is allowed to increase (close continuous system).

Suspension cultures are useful in studying problems related to cell biology including cell cycle and production of secondary metabolites like alkaloids, steroids, glycosides, naphthoquinones, flavones etc. which find medicinal and industrial application. Pharmaceutical industries use large bioreactors for suspension cultures to obtain valuable bioorganic compounds. A bioreactor is a vessel of glass or steel in which cells are cultured aseptically and culture conditions are closely monitored. This results in higher yield of metabolites. In a bioreactor there is provision for adding fresh medium, for harvesting cells, for the aeration of products, for mixing and sampling, for controlling pH, O2 content and temperature Plant cells are immobilised in alginate, agarose, polyacrylamide beads.

Posted Date: 1/24/2013 5:04:13 AM | Location : United States







Related Discussions:- Establishment of suspension cultures, Assignment Help, Ask Question on Establishment of suspension cultures, Get Answer, Expert's Help, Establishment of suspension cultures Discussions

Write discussion on Establishment of suspension cultures
Your posts are moderated
Related Questions
Food infection occurs when a pathogen enters the gastrointestinal tract and multiplies. Microorganisms can penetrate into the intestinal mucosa and grow there, or they can pass thr

What are cotyledons? Cotyledons, or seed leaves, are structures formed by the embryo of angiosperms to absorb nutrients from the endosperm and to keep and transfer these nutrie

Basic structural pattern of limbs The common structural pattern of tetrapod limbs is extremely clear expressed in their skeletal components and in the manner they are organiz

Tissue culture The ability of a plant cell to give rise to a whole plant is called totipotency. The culture of a cell or a tissue in a suitable medium to produce new plan

THYROID GLAND - It develops from the endoderm of the embryo. Th e thyroid gland is the largest endocrine gland located anterior to the thyroid cartilage of the larynx

Q. Moniliasis is one of the most common opportunistic diseases in AIDS. What is the etiological agent of moniliasis and what is the other name of the disease? Why is monilia also c

What are the cells that form the cartilaginous tissue? The main cells of the cartilages are the chondrocytes, originate from the chondroblasts that secrete the intersticial ma

Influence of LV Function :  LV functions, whether normal or impaired is important while selecting a patient for CABG and for long-term results of surgical revascularisation. If le

Describe Pulsation in cardian examination? 1) Apex impulse; lowest and outermost point of definite cardiac pulsation. 2) Prominent pulsations in left parasternal region suggest

Define the metabolism and respiration of cornea. Metabolism and Respiration of Cornea The cornea requires energy for the maintenance of its transparency. Energy in