Establishment of suspension cultures, Biology

Establishment of Suspension Cultures

A callus crumbles into smaller clumps and single cells in liquid medium by gentle agitation (100-120 rPM) on a shaker. Shaking the cultures also helps to aerate the P - cells. Such suspension cultures however rarely comprise single cells alone because cells tend to aggregate in clusters of 2-100. Suspension cultures can be maintained indefinitely by inoculations of known aliquot5 of cells to a fresh medium. This process is termed as "batch cultures". Alternatively, the medium is replenished at regular intervals. This process is termed as "continuous culture". In the continuous culture process at the time of replenishing the medium, cells are also harvested (open continuous system) or the biomass is allowed to increase (close continuous system).

Suspension cultures are useful in studying problems related to cell biology including cell cycle and production of secondary metabolites like alkaloids, steroids, glycosides, naphthoquinones, flavones etc. which find medicinal and industrial application. Pharmaceutical industries use large bioreactors for suspension cultures to obtain valuable bioorganic compounds. A bioreactor is a vessel of glass or steel in which cells are cultured aseptically and culture conditions are closely monitored. This results in higher yield of metabolites. In a bioreactor there is provision for adding fresh medium, for harvesting cells, for the aeration of products, for mixing and sampling, for controlling pH, O2 content and temperature Plant cells are immobilised in alginate, agarose, polyacrylamide beads.

Posted Date: 1/24/2013 5:04:13 AM | Location : United States







Related Discussions:- Establishment of suspension cultures, Assignment Help, Ask Question on Establishment of suspension cultures, Get Answer, Expert's Help, Establishment of suspension cultures Discussions

Write discussion on Establishment of suspension cultures
Your posts are moderated
Related Questions
FLUI D MOSAIC MODEL Proposed by Nicholson and Singer (1972) Proteins and lipids are arranged in a mosaic fashion. Lipids and proteins are in a semi fluid structure.

Alimentary tract The alimentary tract and the secretary organs associated with it are involved in the process of digestion. The sequence of events includes ingestion of food, d

Double helical structure of dna

Explain about the Carbohydrate Malabsorption? Carbohydrates malabsorption is usually caused by an inherited or acquired (in intestinal infection, celiac disease, PEM) defect in

Osmoregulation in Aqueous Environments You are aware that the aqueous environments are of two types: i) Freshwater and ii) Seawater. The osmotic concentration of thes

Write on spoilage of food products by fungi,nutrition,condition for growth,consequencies and prevention.

Q. Which is the kind of muscle tissue that helps to push the food down through the esophagus? The esophageal wall in its superior portion is made of skeletal striated muscle th

Q. Which kind of polarity do fat-soluble and water-soluble substances respectively have? Ans. Water-soluble substances are behave as polar molecules, i.e., they have e

Chronic Mitral Regurgitation Chronic mitral regurgitation may have different aetiological factors: 1) Rheumatic 2) Degenerative-myxomatous malformation 3) Infective

Pseudopodia – Protozoans Pseudopodia are flowing cytoplasmic protrusions of the cell causing amoeboid movement. In protozoans pseudopodia exist in several forms. The most fami