Electron microscopy, Biology

ELECTRON MICROSCOPY (EM)

In EM, the object is viewed by a beam of electrons instead of visible light. An electron beam has a shorter wadelength (h = 0.004 nm) compared with visible light (h = 400 nm). Therefore, an EM has higher resalving power than LM. Till now electron microscopy is the best available method to study biological ultrastructure.

The resolving power of most modem EMS ranges between 100 pm to 0.1 nm. However, problems of specimen preparation, and radiation damage limit the resolution of biological objects to about 2 nm, even this is 100 times better than the resolution achieved by the best LMs. Further, the magnifying power of the EM is upto 200,000 X. The two types of EM are transmission electron microscope (TEM) and scanning electron microscope (SEM). about which you will study in the following section.

Posted Date: 12/10/2012 12:47:35 AM | Location : United States







Related Discussions:- Electron microscopy, Assignment Help, Ask Question on Electron microscopy, Get Answer, Expert's Help, Electron microscopy Discussions

Write discussion on Electron microscopy
Your posts are moderated
Related Questions
Explain Adverse effects of Indinavir   In addition to adverse effects similar to those of other protease inhibitors, indinavir causes elevation of indirect bilirubin, indinavir

Advice on Discharge :  Patients are allowed to go home on 8th or 9th day. It could be even earlier after off pump coronary artery surgery (OPCAB). They should gradually increase t

Water Adaptations After becoming familiar with the two kinds of water stresses you would like to know about the adaptations in plants and animals which enable them to survive u

What are plankton, nekton and benthos? Plankton, nekton and benthos are the three groups into which aquatic living beings may be separated. The plankton is formed by the alg

Air Pollution - Environmental Pollution The threat due to air pollution became apparent only when some severe episodes caused human casualty in USA, Britain (London) and Japan

he recombinant plasmid extracted from the surviving colonies contains a gene called SUC2 that codes for convertase, an enzyme needed to metabolize sucrose. Mutations in the SNF gen

What is mitosis? What is the importance of mitosis? Mitosis is the process in which one eukaryotic cell separates into two cells identical to the parent cell (generally identic

general characteristics of sponges

What are Pure Culture Techniques? Microorganisms can be isolated from various natural environments like soil, food, water, sewage, decomposing matter and even from infectious m

Q. Can you explain Bauhin? We got the first reference of binary use of species name in Pinax (1623), a publication of a Swiss physician and botanist Casper Bauhin (1560-1624).