Dot blot, Biology

Assignment Help:

Dot blot is the technique for measuring the amount of one perticular DNA or RNA in the highly complex mixture. The samples are spotted onto the hybridization membrane (like nitrocellulose or activated nylon, and many more), fixed and hybridized with the radioactive search. The extent of labeling (as determined by autoradiography and the densitometry) is proportional to concentration of the target molecule in its sample. Standards give the means of calibrating the results. 


Related Discussions:- Dot blot

What are epithelial tissues, What are epithelial tissues? What are their ge...

What are epithelial tissues? What are their general function and how is that function associated to the features of the tissue? Epithelial tissues, also known as epithelia, are

What kind of structure is the crystalline lens, Q. What kind of structure i...

Q. What kind of structure is the crystalline lens? What is its function? The crystalline is a converging spherical lens this natural lens has the function to project images of

Acyanotic congenital heart disease, Acyanotic Congenital Heart Disease   ...

Acyanotic Congenital Heart Disease   In this infant has no cyanosis because there is no abnormal communication between pulmonary and systemic circulation or there  is no mixing

How is the concentration of drugs in human plasma defined, Some drugs bind ...

Some drugs bind extensively to plasma proteins (Warfarin binds 99%) while others have virtually no binding. Extraction depends on the type of drug - there are dissimilar standar

Damage to the amygdalas may make a pearson fearless, Damage to the amygdala...

Damage to the amygdalas may make a pearson fearless. Gleb Shumyatsky's lab reported in an article in Cell (2005) that knock out mice [mice lacking a specific gene] lacking the gene

Define foaming properties of proteins, Define Foaming Properties, Binding o...

Define Foaming Properties, Binding of Flavour and Other Substances? To understand the foaming properties of proteins, we need to know some basic aspects of foam foods. Foam foo

Ecological isolation, Ecological isolation is based on the fact that popula...

Ecological isolation is based on the fact that population shows preference to one habitat over the other. This extensive forests become barriers to the dispersal of organims living

What is the initial maintenance steps of implants, Initial Maintenance Step...

Initial Maintenance Steps of implants It is very essential, that from the beginning of the patient interaction regarding the implant therapy, through the diagnosis and planning

What is the cytoskeleton, Q What is the cytoskeleton? What are its major co...

Q What is the cytoskeleton? What are its major constituents in animal cells? Cytoskeleton is the cytoplasmic structure that supports the cell, keeps its fixates and shape and m

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd