Dot blot, Biology

Dot blot is the technique for measuring the amount of one perticular DNA or RNA in the highly complex mixture. The samples are spotted onto the hybridization membrane (like nitrocellulose or activated nylon, and many more), fixed and hybridized with the radioactive search. The extent of labeling (as determined by autoradiography and the densitometry) is proportional to concentration of the target molecule in its sample. Standards give the means of calibrating the results. 

Posted Date: 8/3/2012 6:44:21 AM | Location : United States







Related Discussions:- Dot blot, Assignment Help, Ask Question on Dot blot, Get Answer, Expert's Help, Dot blot Discussions

Write discussion on Dot blot
Your posts are moderated
Related Questions
Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Q. Can the heat capacity of water be considered small or large? What is the biological consequence of that characteristic? From Thermology it is acknowledged that the quantity

Chuck Darwin knew BLENDING THEORY, but he also knew that if it was true his NOT work because

Starch is one of the most common storage products in seeds. What happens to the starch before it can be used by the germinating seed? Starch is insoluble and has to be changed,

What colors of light are needed in a grow light for strawberry development?

Is dwarf pampas grass invasive? No. the root system is shallow and extends only as far as the plant is large. Though if the irrigation system is installed poorly and the pipes

Determine the term - Angiomas Angiomas are congenital collections of abnormal vessels that divert the normal flow of blood. These capillaries, venous, or arteriovenous (A-V) ma

In terms of western medicines, over 3000 antibiotics - including penicillin and tetracycline - are obtained from micro-organisms. Another product of micro-organisms i.e. cyclospori

What are the structures that participate in the human auditory sensitivity? The structures of the human auditory sensitivity are the ears (external, middle and internal), the

If a person has a stomach ulcer (that is aggravated by excessive hydrochloric acid {HCl} secretion ), why on earth would he or she be drinking milk to feel better, when milk is als