Dideoxy sequencing, Biology

Dideoxy Sequencing is the enzymatic determination and consideration of DNA or RNA sequence by the rechnique of Sanger and colleagues, based on incorporation of the chain terminating dideoxynucleotides in a growing nucleic acid strand copied by DNA polymerase or the reverse transcriptase from a DNA or RNA template. Different reactions involve dideoxynucleotides containing A, C, G, or the T bases. The reaction products represent the collection of new labeled DNA strands of the varying lengths, all terminating with the dideoxynucleotide at the 3' end (at site of a complementary base in the template nucleic acid), and are separated in the polyacrylamide or urea gel to generate the sequence "ladder". This technique is more commonly used than the "Maxam-Gilbert" (chemical) sequencing.

Posted Date: 8/3/2012 6:40:31 AM | Location : United States







Related Discussions:- Dideoxy sequencing, Assignment Help, Ask Question on Dideoxy sequencing, Get Answer, Expert's Help, Dideoxy sequencing Discussions

Write discussion on Dideoxy sequencing
Your posts are moderated
Related Questions
On the overall process of cell-to-cell communication within the nervous system, what role does the Ca2+ play in the synapse? A) contributes in the biosynthesis of acetylcholine B)

Determine Teenager mindset and the eating disorders? Self image is an important determinant of weight and figure. Mostly girls under eat as already mentioned earlier, in order

C omp ut e d radiography: The development of intensifying screen has greatly improved the efficiency of exposure  and has reduced the exposure time.   The fir

Causes of Air Pollution We known about the composition of atmosphere, its major and minor constituents. The composition of atmosphere has remained the same for thousands of y

You have learnt about the epidemiology, etiology and pathogenesis of acute rheumatic fever. Revised Jones criteria, which have major and minor criteria, are helping to diagnosis of

To make a 40 mg/mL solution of kanamycin (MW 582.6) how many grams of kanamycine would you dissolve in 500 mL of water?


Temporary Partial Disability - Injury from an Accident Such types of injuries which are easily recoverable and may not incapacitate the victim to perform duty apart from for

Normal 0 false false false EN-IN X-NONE X-NONE

Discuss the biological importance of Imidazole. Bring in examples of biomolecules that contain this group. Please be as thorough as possible.