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Dideoxy Sequencing is the enzymatic determination and consideration of DNA or RNA sequence by the rechnique of Sanger and colleagues, based on incorporation of the chain terminating dideoxynucleotides in a growing nucleic acid strand copied by DNA polymerase or the reverse transcriptase from a DNA or RNA template. Different reactions involve dideoxynucleotides containing A, C, G, or the T bases. The reaction products represent the collection of new labeled DNA strands of the varying lengths, all terminating with the dideoxynucleotide at the 3' end (at site of a complementary base in the template nucleic acid), and are separated in the polyacrylamide or urea gel to generate the sequence "ladder". This technique is more commonly used than the "Maxam-Gilbert" (chemical) sequencing.
Define about the Spectroscopy- colorimetry? Spectroscopy, therefore, is the study of the absorption and emission of radiation by matter. Absorption Spectroscopy is usually used
what is the difination of osmosis
Name some Drugs to prvent tuberculosis Pyrazinamide can cause morbilliform rash, arthralgias and asymptomatic hyperuricemia, and blocks the hypouricemic action of allopurinol (
Acute Bronchitis: Acute bronchitis is inflammation of one or more bronchi and occurs specifically in children younger than four year?. It is usually associated with previ
Somatic Embryogenesis The process of embryo development is called embryogenesis. It is not the monopoly of the egg to form an embryo. Any cell of the female gametophyte (Embr
Plate Tectonic Is a Theory of Geology Which describes the large scale motion of earth's lithosphere. The theory builds on the older theory of continental drift from the first half
Ecosystem as a Unit of Nature An ecosystem can be visualised as a functional unit of nature representing complex interactions between living and non-living components. Th
what is a synapse and what does it do?
Give an example of how two organisms are interdependent.
Q. Emergency treatment of diarrhoea? Emergency treatment and drug treatment: Severe dehydration is fatal and requires intravenous fluids and hence hospitalization. You have re
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