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For many years, students who have occupied my Molecular Biology course have now applied for lab tech jobs exact after graduation. But I have NEVER had a student fail to obtain a job before graduation. One purpose for this is that my all students learn to create solutions. Four problems are here which you could remain off the unemployed college graduate list.
a) You obtain an optical density reading of a DNA sample but this is too concentrated to provide you an accurate reading. You obtain 1 μL of the DNA sample and dilute this with 14 μL of dH2O. You after that take a reading of the DNA onto the spectrophotometer and this provide you an OD reading of 0.123. As written above the spectrophotometer is such above conversion factor: 1 OD of ds DNA = 50 μg/mL of DNA.
Question: Determine the concentration of your original DNA sample?
b) You require preparing a few solutions for a Southern blot. You are provided 50X SSC, 10 percent SDS as well as 5 M NaOH.
Question: Explain how would you make 250 mL of a solution those final concentrations were 1X SSC, 0.25 percent w/v SDS and 10 mM NaOH? When FW of NaOH is 40.0.
The complications that occur in the heart due to hypertension are left ventricular hypertrophy, diastolic dysfunction and cardiac failure. There is also associated coronary artery
What is pollination? What are the main forms of pollination? The process in which pollen grains (the male gametophytes of phanerogamic plants) reach the female gametophyte is k
What are presbyopia and astigmatism? Presbyopia is the visual impairment in which there is loss of the cililary muscle strength therefore reducing the capability of the crystal
Considering hybridization in a trait like the color of the flowers of a given plant species (red/yellow) conditioned by a pair of different alleles in relation to complete dominanc
Classification of coelentrata upto sub classes
Problem 1: How many different types of RNAs are present? What is the function of Promoter in Transcription? Mention different types of RNAs Discuss function of Promot
To which direction does the growth of one side of a stem, branch or root induce the structure to curve? While one side of a stem, branch or root grows more than the other side
Q. How does synaptic transmission between neurons take place? The propagation of the action potential along the axon reaches the region immediately anterior to the presynaptic
1. A man with normal pigment, whose father was an albino, marries an albino. What chance is there that their first child will be an albino? 2. A man with normal pigment marries
Q. Is pollution always caused by humans? In the most cases pollution is caused by human activity. Other species and some abiotic factors though can also pollute an ecosystem. F
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