Design of expression vectors in transgenic animals, Biology

Design of expression vectors

Expressing a transgene in an appropriate manner is a huge task. The gene construct that is quite active in transfected cells may be only weakly expressed in transgenic animals. Moreover, each line of animals shows a specific pattern of transgene expression. In addition, a number of transgenes remain silent in some cells but are expressed in others. This is a clear limitation for the use of transgenic animals. A large number of transgenic founders are attempted until they find the lines expressing the foreign gene as they expected. This approach implies wastage of time and animals but it appears sufficient to obtain 1,000-10,000 copies of recombinant protein per cell exhibiting the expected biological action. This relative but real success of the expression vectors dissuaded researchers for years from improving them.

The situation is altogether different when recombinant proteins are to be prepared in milk. Highly efficient expression vectors are often needed to obtain the expected large amounts of proteins recovery from milk. However, it must be ensured that the recombinant proteins are restricted to milk as much as possible to avoid any potential deleterious effects on host animals. A significant amount of work was done for the production of recombinant proteins in milk and a few efficient expression vectors were designed and patented. Yet basic studies on the mechanisms of gene expression and empirical observations have led to defined rules for vector con­struction and to identification of pitfalls that can be avoided. A functional transcription unit is composed of several elements that cooperate with each other. These elements and their mode of action are not yet fully known. A gene construction consists of associating more or less known elements. This may generate nonfunctional vectors for unknown reasons. The major recommendations for design­ing reliable vectors may be summarized as follows.

Posted Date: 9/18/2012 7:42:23 AM | Location : United States







Related Discussions:- Design of expression vectors in transgenic animals, Assignment Help, Ask Question on Design of expression vectors in transgenic animals, Get Answer, Expert's Help, Design of expression vectors in transgenic animals Discussions

Write discussion on Design of expression vectors in transgenic animals
Your posts are moderated
Related Questions
Q. What is the neurotransmitter of the neuromuscular junction? How does the nervous system trigger muscle contraction? The nervous cells that trigger the muscle contraction are

Central Nervous System   Hypertension is one of the leading causes of cerebrovascular disease. It has been associated with accelerated age related cognitive decline. The most d

Illustrate the diabetic contract of the eye? Diabetic Cataract: 1) Sorbitol Aldose Pathway: In patients with diabetes, there is increase in sugar alcohol which results in

Briefly Describe about Development of Sexual Maturity in Children? Sexual maturity develops along with growth spurt in adolescence. In girls, growth stops on attaining menarche

SPERMEIOGENESI S / SPERMETOLEOSIS It is the gradual differentiation of a stationary rounded and undifferentiated spermatid into an active and motile sperm. The spermatids f

Q. What are the environmental harms caused by mercury pollution? What are the main sources of mercury pollution? The Mercury is a metal that when present in the water of rivers

How to Calculate the Biological Value of Protein? A method for determining the biological value of proteins was developed by Mitchell in 1925. It measures the quantity of dieta

Explain about the Thiamin - Vitamin B or aneurin? Thiamin is one of the earliest recognized vitamins. The chemical structure of thiamine was established by Williams in 1936. Th

Define the neurological disorders of dysphagia? In neurological disorders, damage to cranial nerves can lead to weak and poorly co- ordinated tongue movements, which results in

Which is the extraembryonic membrane whose function is to store nitrogen wastes of the embryo? Is this function present in placental mammalian embryos? The allantois is the ext