Denaturing gel, Biology

Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel. 

Posted Date: 8/3/2012 6:39:45 AM | Location : United States







Related Discussions:- Denaturing gel, Assignment Help, Ask Question on Denaturing gel, Get Answer, Expert's Help, Denaturing gel Discussions

Write discussion on Denaturing gel
Your posts are moderated
Related Questions
Give an example of how two organisms are interdependent. Animals generate carbon dioxide and water, which is required by plants. Accept all reasonable answers.

He p atitis Hepatitis is defined as degenerative/ inflammatory processes of the liver. The clinical manifestations associated with hepatic dysfunctions results from impairmen

organism that can not exist in the presence of oxygen ?

Energy Flow - Ecosystem Our world is a solar-powered system, and green plants are the entry gates of energy into ecosystem. The total incoming solar energy, only a very small

Alkaline Phosphatase is an enzyme which catalyzes the hydrolysis of the phosphomonoesters of the 5' nucleotides. It is used to dephosphorylate (remove phosphate groups from) the 5

Lipase Pancreatic lipase specifically  hydrolyses the primary ester linkages i.e.  at the position 1 and 3 of triacylglycerols. The presence of bile salt helps  in  emulsificat

What is the best microscope to get a detailed view of the parts inside of a preserved plant cell? Transmission electron microscope Scanning electron microscope Compound li

Define observation or inference for barfoed test? 1. Reddish brown precipitate is seen on the sides and bottom of the tube. The precipitate of the sides and bottom indicates th


Extracellular Aging The basic components of extracellular space are mucopolysaccharides and fibrous proteins, particularly collagen and elastin. These proteins are synthesised