Define ziehl-neelsen staining technique, Biology

Assignment Help:

Define Ziehl-Neelsen Staining Technique?

Ziehl-Neelsen staining is used for staining these bacteria. The technique was discovered first by Robert Koch during his pioneering investigations on tuberculosis and was the fore-runner of Ziehl-Neelsen stain used today for diagnosis of these organisms in laboratory. Acid fast bacteria are called so because once these cells get stained with basic fuchsin, these resist decolourization with acid alcohol as can be seen in margin illustiation. This acid fastness red stain is due to high lipid content particularly of mycolic acid in their cell wall. Mycolic acid, is a group of branched chain hydroxyl lipids, which bind covalently to peptidoglycan of cell wall giving the cell surface a waxy, hydrophobic consistency.


Related Discussions:- Define ziehl-neelsen staining technique

draw the cell at metaphase, Consider cell with a diploid chromosome number...

Consider cell with a diploid chromosome number of 4 (2n=4), one pair of the chromosomes is metacentric,the other is acrocentric. The metacentric is labeled a 1 a 1 while the acr

The oxidative phase generates nadph, 1)  The oxidative phase  generates N...

1)  The oxidative phase  generates NADPH The oxidative branch of  the pathway  generates NADPH  and pentose-5-phosphate, through the following  reactions: a)  Glucose-6-pho

What is the average duration of each stage in hours, A biologist examines a...

A biologist examines a series of cells and counts 140 cells in interphase, 10 cells in metaphase, 4 cells in anaphase and 7 cells in telophase. if complete cell cycle requires 24 h

Endomyocardial biopsy, Endomyocardial biopsy helps in understanding that ca...

Endomyocardial biopsy helps in understanding that carditis can cause CCF in patients with rheumatic heart disease but frequency of diagnostic features on EMB is found only in 17 pe

Explain therapeutic diet planning, Explain therapeutic diet planning Al...

Explain therapeutic diet planning All detailed dietary modifications  should be presented with choices,  clear guidelines, menu guidance and supporting  information  as  to  al

Determine the concept of imitation in the learning process, Determine the c...

Determine the concept of  imitation in the learning process The concept of  imitation in the learning process is also a key developmental concept for infants and young children

Enumerate the major substrates for gluconeogenesis, Enumerate the major sub...

Enumerate the major substrates for gluconeogenesis The major substrates for gluconeogenesis are the glucogenic amino acids, lactate  glycerol and (important  in ruminant) propi

Principles governing cleavage, Principles Governing Cleavage a) The n...

Principles Governing Cleavage a) The nucleus and mitotic achromatic figure tend to occupy the centre of active cytoplasmic density of the dividing cells, e.g., in isolecithal

Gene expression is controlled by chromatin state, Gene expression is contro...

Gene expression is controlled by chromatin state, _______ and _________. a. transcription factors and epigenetic regulators b. polymerases and control proteins c. delta and tau pro

Fracture and its various types, Fracture - It is a break of a bone. Bone f...

Fracture - It is a break of a bone. Bone fracture are of many types - (i) Green stick fracture - It is merely a crack, the bone remains partly intact. (ii )     Sim

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd