Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define the Principle behind Cyanmethemoglobin Method?
The principle behind the cyanmethemoglobin method is as follow:
Principle The haemoglobin is treated with a reagent containing potassium cyanide and sodium carbonate. With haemoglobin ferricyanide forms methaemoglobin which is converted to cyanmethaemoglobin or haemiglobincyanide (HiCN) with cyanide. The absorbance is measured at 540 nm. The reaction is presented herewith:
Haemoglobin + potassium ferricyanide ------→ Methaemoglobin
Methaemoglobin + potassium cyanide ------→ Cyanmethaemoglobin (HiCN)
in solution the ferrous ions of the haemoglobin are oxidized to the ferric state by potassium fericyanide to form methaemoglobin. In turn methaemoglobin reacts with cyanide ions (CN-) provided by potassium cyanide to form HiCN. The time required for full colour development is shortened by if dihydrogen potassium phosphate is substituted for sodium bicarbonate in the classic Drabkin's reagent. The colour is stable for more than 24 hours in well stoppered tubes kept in dark. An HiCN standard can be used to calibrate the spectrophotometer, The concentration of HiCN in the sample is calculated from the absorbance in a calibrated spectrophotometer or alternatively with the help of a standard curve.
Q. Why isn't the cooking of vitamin C-containing foods appropriate for vitamin C supply? To obtain vitamin C, for instance, from an orange dessert, the vitamin- containing food
What is Strobilization. Elaborate. The process which converts scyphostome into a strobila during the life cycle of schyphozoans, jellyfish. Transverse divisions of strobila pro
Q. Symptoms of inflammatory bowel disease? Inflammatory bowel diseases are associated with: • Abdominal cramping, diarrhoea • Steatorrhoea • Obstruction caused due t
Normal 0 false false false EN-IN X-NONE X-NONE
Explain in details Aerobic Respiration? The pyruvate produced in glycolysis is further broken down in most organisms. This process, called aerobic respiration, requires gaseou
What is the functional unity of the kidneys? The functional (filtering) unity of the kidneys is the nephron. A nephron is made of afferent arteriole, efferent arteriole, glomer
#qcarrieruestion..
Indication : IABP is commonly used to supporting failing left ventricle after open-I1ca1-t surgery or in cardiogenic shock after myocardial infarction. It is at times used in uns
NUMBER Benden and Boveri first indicated that number of Chromosomes is definite in each organism. (1) Haploid (n) - One set of Chromosomes. i.e. one Chromosome of each
Freshwater Animals In Figure you have seen that the body fluids of freshwater animals are hyperosmotic to their aqueous surroundings. This results in two-kinds of osmotic prob
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +1-415-670-9521
Phone: +1-415-670-9521
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd