Cytochemistry, Biology

Cytochemistry

It is well known that certain stains and dyes specifically combine with certain chemical components of the cell. Cytochemical techniques take advantage of this fact and help in localisation and identification of various constituents like proteins, nucleic acids, lipids, etc. within a cell. For this a particular molecule is first immobilized and then identified by its reaction with a stain. Some molecules are also identified by their physical properties like absorptian of radiation at a particular wavelength. For example, the nucleic acids absorb UV rays at 260 nm whereas the proteins absorb at 280 nm.

The amount of the chemical component present in a cell can be quantified provided the reaction betwzen the molecule present and the stain is strictly on 1: 1 basis, as in the case of Feulgen reaction for DNA explained.

A large number of dyes are in use to stain different chemical components of the cell.However, the chemical basis for the specificity of many dyes is not known, so only qualitative information can be obtained.

Posted Date: 12/10/2012 12:35:22 AM | Location : United States







Related Discussions:- Cytochemistry, Assignment Help, Ask Question on Cytochemistry, Get Answer, Expert's Help, Cytochemistry Discussions

Write discussion on Cytochemistry
Your posts are moderated
Related Questions
Water Water usually forms about 50% to90% part of living protoplasm. Life upon earth originated in the ocean and the conditions of that aqueous primeval environment have been r

Define Active or moderately active Lifestyles - physical activity? These people have occupations that p-e not strenuous in terms of energy demands, but involve more energy expe

What are melanocytes? Melanocytes are epithelial cells of the skin particular in secretion of melanin. Melanin is a pigment that besides coloring the skin, the iris of the eye

Sutures are also classified by thread diameter: Thread materials range in diameter from 1 to 10 with the higher number corresponding to the thinner, more delicate thread. In

Goat pox Goat-pox is common in occurrence, and its nature is similar to sheep pox. The incubation period varies from 5 to 10 days. The disease tends to attack male kids and ewe

Q. Do echinoderms have circulatory and respiratory systems? In echinoderms there are not well- defined respiratory with the exception of the holothurian group and circulatory s

classificationof algae

write down the division of cryptogamae and phanerogamae

Provide supporting evidence to explain how conversion of a normal somatic cell into a malignant one requires multiple mutations.

Define the Bioavailability of Vitamin K? Very little is known about the bioavailability of the K vitamins from different foods.  It has been estimated that the efficiency of ab