Culture conditions, Biology

Culture Conditions

The culture vessels placed in suitable trays or racks, are stored on the shelves in a hygienically maintained culture room, under controlled conditions of light and temperature. If possible the relative humidity should not fall below 50% to prevent rapid desiccation of the medium. To minimize the infection of cultures during incubation, the culture room should be dust free. It is often provided with double doors and entry of people is restricted. The requirement for light and temperature varies considerably. The cultures of high altitude plants as well as those of desert species grow well at 25? c-28?c. However, induction of pollen embryogenesis requires a high temperature treatment (35? C). As mentioned earlier, in cultures even green tissues and shoots do not exhibit active photosynthesis and are largely heterotrophic. The differentiation of shoots from callus or explants and axillary shoot proliferation are favored by light.

A callus is defined as an unrecognized mass of tissue varying widely in texture, appearance and growth rate. Such cultures should be maintained in continuous diffuse light (1-3K lux) provided by fluorescent tubes. You should know that unorganized callus and freshly isolated protoplast cultures are often sensitive to light and are incubated in darkness. Total darkness is also favorable for rooting and for the initial stages of pollen and somatic embryogenesis.

Posted Date: 1/24/2013 5:02:44 AM | Location : United States







Related Discussions:- Culture conditions, Assignment Help, Ask Question on Culture conditions, Get Answer, Expert's Help, Culture conditions Discussions

Write discussion on Culture conditions
Your posts are moderated
Related Questions
Define Requirements of Fat in Postoperative Nutritional Care Adequate amount of fat is needed to build up and maintain tissue fat reserves. Depending upon the existing health a

Keel is characteristic of the flowers of : 1. Gulmohur 2. Cassia 3. Calotropis 4.  Bean Bean

BRONCHUS - In thorax trachea is devided in to 2 bronchi that enter into lungs of its own side. Rings present in it. BRONCHIOLES - These are branches of bronchus.

Determine about the scales developed by golden Several other scales have been developed by Golden and various collaborators, all of which are based on different ways of scoring

Explain about the Acesulfame K - Artificial Sweeteners? Acesulfame-K (potassium salt of 6-methyl-1, 2, 3-oxathazin-4(3H)-one 2, 2 dioxide) is a sweetener which is commonly used

Why do fats have thermal isolation properties? Triglycerides are weak heat conductors and in addition they form thick layers of fat tissue when accumulated by the organism. Tha

Social Status and Support Network Income and Social Status: Higher income and social status lead to better health.Employed persons, having more control over their workin

Pound cake test In some cases, oil or margarine creaming volume is most accuratelymeasured by preparing a regular pound cake, omitting the chemical leavener andmeasuring the vo

Downer cow syndrome This syndrome is a common sequel to milk fever. The term is commonly applied to those hypocalcaemic cows that remain recumbent for several hours before bein

Aid-giving behaviour between genetically related individuals in animal groups is often observed in nature. This aid-giving behaviour can evolve when the advantages of helping relat