Composition - culture medium, Biology

Composition - Culture Medium

A standard plant tissue culture medium (Basal medium) contains all the essential macro elements (Carbon, hydrogen, oxygen nitrogen, phosphorus, sulphar, calcium, potassium and magnesium) and iron and microelements(iron, manganese, copper, zinc, boron and molybdenum) but the concentration of their salts in different formulations vary. In addition some vitamins and sucrose (2-3%) are universal constituents of plant tissue culture media. The composition of Ms medium (Murashige and Skoog's) medium found satisfactory for a wide range of monocotyledonous and dicotyledons species.

Besides the nutrients, one or more plant growth regulators (PGR7S) are generally required for supporting good growth of the cultured material. The PGR'S most widely used in tissue culture media are auxins (2, 4-D, IAA, NAA, IBA) and cytokinins (BAP, Kinetin). The PGR7s are particularly important for the growth of Callus tissues and organogenic embryogenic differentiation. Usually the medium is gelled with 0.8% bacteriological agar.

Posted Date: 1/24/2013 4:49:31 AM | Location : United States







Related Discussions:- Composition - culture medium, Assignment Help, Ask Question on Composition - culture medium, Get Answer, Expert's Help, Composition - culture medium Discussions

Write discussion on Composition - culture medium
Your posts are moderated
Related Questions
What are some prophylactic measures for tapeworm infections? The main prophylactic measures against taeniasis and cysticercosis are: not to ingest raw or badly cooked swine or

Q. Evolutionarily protochordates may be intermediate animals between invertebrates and vertebrates. Imagine a scientist is testing the hypothesis that vertebrates evolved from echi


Work Injuries Based On Type of Accidents Earlier we identified four kinds of injuries mainly based upon type of disability. This classification is needed for the purposes of c

General characters of phylum protozoa

Q. In chemical terms how is the neuronal repolarization achieved? Repolarization is the return of the membrane probable from the action potential (+35 mV) to the resting potent

Q. What are the main symptoms of the pulmonary and of the intestinal phases of the ascaris infestation? In the pulmonary period the ascaris infestation causes hemoptysis, cough

Implementation of Nursing Care   Prepare the Patient for Diagnostic Evaluation Your role as a nurse is to provide continuing support to the child and family during diagnos

The Peripheral membrane proteins are less strongly bound to the lipid bilayer than integral   membrane   proteins   and can be readily   erased   through washing   the membranes wi

Animal and Plant Cells The internal structural organisation and information related with hereditary characters and metabolic pathways are similar in all eucaryotic cells whethe