Composition - culture medium, Biology

Composition - Culture Medium

A standard plant tissue culture medium (Basal medium) contains all the essential macro elements (Carbon, hydrogen, oxygen nitrogen, phosphorus, sulphar, calcium, potassium and magnesium) and iron and microelements(iron, manganese, copper, zinc, boron and molybdenum) but the concentration of their salts in different formulations vary. In addition some vitamins and sucrose (2-3%) are universal constituents of plant tissue culture media. The composition of Ms medium (Murashige and Skoog's) medium found satisfactory for a wide range of monocotyledonous and dicotyledons species.

Besides the nutrients, one or more plant growth regulators (PGR7S) are generally required for supporting good growth of the cultured material. The PGR'S most widely used in tissue culture media are auxins (2, 4-D, IAA, NAA, IBA) and cytokinins (BAP, Kinetin). The PGR7s are particularly important for the growth of Callus tissues and organogenic embryogenic differentiation. Usually the medium is gelled with 0.8% bacteriological agar.

Posted Date: 1/24/2013 4:49:31 AM | Location : United States







Related Discussions:- Composition - culture medium, Assignment Help, Ask Question on Composition - culture medium, Get Answer, Expert's Help, Composition - culture medium Discussions

Write discussion on Composition - culture medium
Your posts are moderated
Related Questions
CLASSIFICATION OF GENERAL DRUGS - 1 .       NATURAL DRUGS - Obtained from natural sources as minerals, animals, plants & microorganism. (i)       Minerals - It include

Can you please describe the differences between continuous traits and discrete traits and how it helps an ecologist measure variation? Please give a few examples.

Pressure and Continuity Pressure Water pressure increases with depth which varies in the ocean from 1 atmosphere where at the surface to 1000 atmosphere at the greatest

Explain Exclusion diets Exclusion diets: Specific dietary exclusion becomes a necessity  in  case of  food allergy or food intolerance.  The therapeutic use of  such  diets req

phase of alpha taxonomy

THEO R Y OF PANGENESIS - Proposed by Charles Darwin. According this miniature present in the every body cell i.e. called gemmule

Explain the term- Horizons These processes take a long time- may be a few thousand years to create a soil. As the time passes, the soil matures and generally becomes deeper and

Which of Mendel's postulates can only be demonstrated in crosses involving at least two pairs of traits? a) Segregation b) dominance/recessiveness c) independent assortment d) unit

Q. What is the vector of Chagas' disease? How is the disease transmitted? The vector of the Chagas' disease is its intermediate host, a triatomine bug. The key species is Triat