Composition - culture medium, Biology

Assignment Help:

Composition - Culture Medium

A standard plant tissue culture medium (Basal medium) contains all the essential macro elements (Carbon, hydrogen, oxygen nitrogen, phosphorus, sulphar, calcium, potassium and magnesium) and iron and microelements(iron, manganese, copper, zinc, boron and molybdenum) but the concentration of their salts in different formulations vary. In addition some vitamins and sucrose (2-3%) are universal constituents of plant tissue culture media. The composition of Ms medium (Murashige and Skoog's) medium found satisfactory for a wide range of monocotyledonous and dicotyledons species.

Besides the nutrients, one or more plant growth regulators (PGR7S) are generally required for supporting good growth of the cultured material. The PGR'S most widely used in tissue culture media are auxins (2, 4-D, IAA, NAA, IBA) and cytokinins (BAP, Kinetin). The PGR7s are particularly important for the growth of Callus tissues and organogenic embryogenic differentiation. Usually the medium is gelled with 0.8% bacteriological agar.


Related Discussions:- Composition - culture medium

Trace minerals-chromium, Chromium Chromium functions as a component of ...

Chromium Chromium functions as a component of the glucose tolerance factor, which serves to potentiate the action of insulin. Adding low concentrations (0.02 to 1.0 mg/kg) of c

Genetically modified foods-vaccines, Normal 0 false false f...

Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4

Plankton - aquatic ecosystem, Plankton - Aquatic Ecosystem This group ...

Plankton - Aquatic Ecosystem This group includes both microscopic plants (phytoplankton) and animals (zooplankton) found in all aquatic ecosystems, except certain swift moving

Nutritional requirement and dietary modifications in diet, Define Nutrition...

Define Nutritional Requirement and Dietary Modifications in the Diet of the Elderlu? The nutritional status influences the age-related rate of functional decline in many organ

Peptide bond formation, Peptide bond formation is catalyzed by peptidyl tra...

Peptide bond formation is catalyzed by peptidyl transferase.  The carboxyl  end of the amino acid bound to the tRNA in this reaction and in the P site is uncoupled  from the tRNA a

Role of hormones in aging, Role of Hormones in Aging Aging as well ref...

Role of Hormones in Aging Aging as well reflects the inefficiency of the endocrine system. A change in the hormonal secretions may contribute to the development of physical ai

What do you mean by alpha diversity, Q. What do you mean by Alpha diversity...

Q. What do you mean by Alpha diversity? Alpha (a) richness refers to the number of species found in small, homogenous area. Alpha diversity refers to the diversity in a partic

Morphological differences between monocot and dicot plants, Q. What are the...

Q. What are the major morphological differences between monocot plants and dicot plants? The main separation criteria between monocots and dicots are: number of cotyledons (see

List four common exercises you will recommend to a patient, List four commo...

List four common exercises you will recommend to a patient. Common exercises are: - brisk walking - cycling - swimming - playing tennis - dancing - rope skip

Pericardium, The heart is enclosed in a membranous sac called the pericardi...

The heart is enclosed in a membranous sac called the pericardium. It has two layers- the fibrous pericardium which is the outer layer and the serous pericardium that lies inside th

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd