Chromosome walking, Biology

Chromosome walking is the technique for cloning everything in genome around the known piece of DNA (the begning probe). You screen the genomic library for all the clones hybridizing with probe, and then figure out which one extends furthest into surrounding DNA. The most distal segment of this most distal clone is then taken in use as a probe, so that ever more distal areas can be cloned. This has been taken in use to move as much as 200 kb away from the given begning point (an immense undertaking). In general it is used to "walk" from a starting point towards some nearby gene in order to clone that specific gene. It is also used to obtain the remainder of a gene when you have isolated a part of it.

Posted Date: 8/1/2012 7:18:28 AM | Location : United States







Related Discussions:- Chromosome walking, Assignment Help, Ask Question on Chromosome walking, Get Answer, Expert's Help, Chromosome walking Discussions

Write discussion on Chromosome walking
Your posts are moderated
Related Questions
why should breathe faster after a hard work


In studies of human body, which of the subsequent terms can also be used when referring to the pituitary gland? Is it: a) Pancreas b) Hypozthesis (pron: hypo-fi-sis) c) T

Determine about the Sense of  Contrast It is  the ability to  perceive  slight changes  in  luminance between  regions which  are not  separated by  definite borders. This  is

Explain the role of calcium ions, troponin and F-Actin during contraction in striated muscles of humans?

Fat digestion requires two steps? What are the steps and what enzymes are used to accomplish each step?

Define Signs and symptoms of iron deficiency anaemia? Since the level of haemoglobin is reduced in the blood, it causes paleness (pallor) on certain parts of the body. Initiall

The excretory organs and excretory materials of plants and animals

Consider Neuron B in the frog central nervous system whose plasma membrane has a previously unknown channel that is selectively conductive to a newly discovered tetravalent anion n