Chromosome jumping, Biology

Chromosome jumping is the technique whereby one begins with a piece of DNA from one area of a chromosome, and obtains clones from nearby areas without cloning everything in between (as in chromosome walking). One round of jumping yields the new clones at the distances of several tens of kb away from the beginning point. In practice, this method is used when classical genetics proves that a known piece of DNA is located on the chromosome close to the gene you would like to clone (like a human disease gene). By cloning the fragments some distance away in both the directions from the known fragment, one might attain 
(1) fragments further from the required gene (which are discarded); 
(2) the fragments are even more closely linked to the required gene (in which case one goes for another round of jumping); or 
(3) fragments from within required gene - the optimal result. 

Posted Date: 8/1/2012 7:17:41 AM | Location : United States







Related Discussions:- Chromosome jumping, Assignment Help, Ask Question on Chromosome jumping, Get Answer, Expert's Help, Chromosome jumping Discussions

Write discussion on Chromosome jumping
Your posts are moderated
Related Questions
Viral haemorrhagic fevers (VHF) Viral haemorrhagic fevers (VHF) are caused by a diverse group of viruses belong to the families Arenaviridae, Bunyaviridae, Filoviridae and Fla

The destruction of the ozone layer may be responsible for an increase in

Determine energy expenditure for Climbers? The energy expenditure of 3250 Kcal/ day is reported in climbers to Mt. Everest using doubly labeled water teaching. Out of this, 161

How is lymph propelled through the lymphatics? Some of the larger lymphatics are capable to contract, or else the lymph is propelled by body muscles which contract and 'squash'

STRUCTURE Golgi body consists of :- (1 ) Cisternae These are plate like unbranched structure with dilated ends. One golgi body has 4-8 cisternae. Mono cisternal

Can enhancers work in Trans? That is, can an enhancer on one piece of DNA activate a promoter on another piece of DNA? Propose an experiment to test this. Be sure to discuss any ap

various changes that occur during concentration of food

Q. Predominant feature of steatorrhoea? A predominant feature is delayed and defective absorption of fat, which results in bulky stools containing large quantities of fat (know

PADP may be determined by measuring the end diastolic velocity of PADP = 4 V 2 Pulmonary regurgitant signal and applying the following formula V is  the end diastolic veloci

Health Dimensions Of Development It is widely recognised that the health status of the people of a country is as important as their economic status. The role of the governm