Chlamydiosis-diagnosis, Biology

Diagnosis

Clinical signs: In most of the cases in initial stages like bacterial infections there is leucocytosis with neutrophilia, which at later stages of the disease may convert to leucocytosis with lymphocytosis. There is slight change in albumin – globulin ratio and also in total protein Other manifestations like abortions/ still births/ premature births, fever, pneumonia/eneritis etc. may also be noticed.


Cytological studies: Recognition of elementary bodies and/or cytoplasmic inclusions in impression smears, scraping or exudates or fluids stained by modified Giemsa or Giemenz methods.

Isolation of specific agents is done from discharges, blood, faeces or postmortem tissues of dead animals in 6-7 day-old chick embryos through yolk sac route or in mice or in guinea pigs through intra peritoneal route or footpad inoculations.Serology plays an important role in the diagnosis of latent or chronic infections as well as in the confirmation of on-going diseases. The most efficient serological method of the diagnosis is the complement fixation test (CFT), agar gel diffusion test (AGDT), agglutination test, serum neutralization (SN) and haemagglutination inhibition (HI) test. Enzyme linked immunosorbant assay (ELISA) is being used routinely for diagnosis of infection. The immunofluorescent test (IFT) is however the most sensitive than any other diagnostic tests and is utilized for confirmation. In addition, immuno-peroxidase and immuno-histochemical tests are also used for the diagnosis. Electron microscoples (E/M), polymerase chain reaction (PCR), nucleic acid hybridisation (NAH) are the recent biotechnological tools being used in the diagnosis of Chlamydial infection.

Posted Date: 9/19/2012 1:34:46 AM | Location : United States







Related Discussions:- Chlamydiosis-diagnosis, Assignment Help, Ask Question on Chlamydiosis-diagnosis, Get Answer, Expert's Help, Chlamydiosis-diagnosis Discussions

Write discussion on Chlamydiosis-diagnosis
Your posts are moderated
Related Questions
What are sensory receptors? Sensory receptors are structures specialized in the acquiring of information, such as temperature, mechanical pressure, pH, chemical environment and

What Do We Mean By Culture Media? Culture media, you would realize, is a solid or liquid preparation containing all the nutrients required by microbes for growth. So then, what

What is meant by the arch reflex? In some situations the movement of the skeletal striated muscles does not depend upon commands of the superior motor neurons, i.e., it is not

Regeneration - Root and Shoot Morphogenesis Higher plants have three kinds of regenerative activities; All plants possess inherent ability to regenerate lost parts. Fo

What is market in management term? A market: There is Buyers and sellers for a good or a service come within contact for the function of normally exchange, for money. Lik

Explain about the Canning of Food? You must have seen the markets flooded with canned products. Can you name a few of such products? Yes the canned juices, canned aerated drink


What are the main cytoplasmic structures present in animal cells? Ans) The major cytoplasmic structures of the cell are the centrioles, the cytoskeleton, lysosomes, mitochondria

A common biocontrol agent for the control of plant diseases is : 1. Baculovirus 2. Bacillus thuringiensis 3. Glomus 4. Trichoderma Trichoderma

Discuss in brief about maxillary sinus In normal conditions, the maxillary sinus maintains a close relationship with the alveolar ridge associated with second premolar and fir